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Literature summary for 2.1.1.6 extracted from

  • Wang, Q.; Teng, M.; Li, X.
    Functional and structural characterization of a novel catechol-O-methyltransferase from Schizosaccharomyces pombe (2019), IUBMB Life, 71, 330-339 .
    View publication on PubMed
Search for more literature for 2.1.1.6

Protein Variants

Protein Variants Comment Organism
D162A loss of S-adenosylmethionine binding Schizosaccharomyces pombe
E106A loss of S-adenosylmethionine binding Schizosaccharomyces pombe
H163A loss of S-adenosylmethionine binding Schizosaccharomyces pombe
I56A dissociation constant of S-adenosylmethionine is similar to wild-type. Residue I56 forms a pair of H-bonds with oxygen in the methionine portion Schizosaccharomyces pombe
S86A loss of S-adenosylmethionine binding Schizosaccharomyces pombe
V107A significant decrease in S-adenosylmethionine binding Schizosaccharomyces pombe
W164A dissociation constant of S-adenosylmethionine is similar to wild-type. Residue W164 forms a T-shaped pi-pi interaction with the adenine ring Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 esculetin pH 7.5, temperature not specified in the publication Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolutely required Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe O42898
-
-
Schizosaccharomyces pombe ATCC 24843 O42898
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + esculetin
-
 Schizosaccharomyces pombe S-adenosyl-L-homocysteine + scopoletin
-
 ?
S-adenosyl-L-methionine + esculetin
-
 Schizosaccharomyces pombe ATCC 24843 S-adenosyl-L-homocysteine + scopoletin
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0289
-
 esculetin pH 7.5, temperature not specified in the publication Schizosaccharomyces pombe

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.105
-
 esculetin pH 7.5, temperature not specified in the publication Schizosaccharomyces pombe