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Literature summary for 2.1.1.57 extracted from

  • Chen, Y.; Su, C.; Ke, M.; Jin, X.; Xu, L.; Zhang, Z.; Wu, A.; Sun, Y.; Yang, Z.; Tien, P.; Ahola, T.; Liang, Y.; Liu, X.; Guo, D.
    Biochemical and structural insights into the mechanisms of SARS coronavirus RNA ribose 2-O-methylation by nsp16/nsp10 protein complex (2011), PLoS Pathog., 7, e1002294.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.57

Activating Compound

Activating Compound Comment Organism Structure
nsp10 protein nsp16 requires non-structural protein nsp10 as a stimulatory factor to execute its methyltransferase activity by binding both S-adenosyl-L-methionine and RNA substrate Severe acute respiratory syndrome-related coronavirus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Severe acute respiratory syndrome-related coronavirus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using in 0.1 M MES, pH 5.0, 2 M NaCl, 0.1 M NaH2PO4, 0.1 M KH2PO4 after 24 h at 25°C Severe acute respiratory syndrome-related coronavirus

Protein Variants

Protein Variants Comment Organism
G70A the mutant shows reduced activity compared to the wild type Severe acute respiratory syndrome-related coronavirus
H83A/P84A inactive Severe acute respiratory syndrome-related coronavirus
K93A the mutant shows reduced activity compared to the wild type Severe acute respiratory syndrome-related coronavirus
Y76A/C77A/R78A inactive Severe acute respiratory syndrome-related coronavirus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus G8H306 nsp16
-
Severe acute respiratory syndrome-related coronavirus WHU G8H306 nsp16
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and Superdex 200 gel filtration Severe acute respiratory syndrome-related coronavirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not methylate m7G(5')pppG-RNA Severe acute respiratory syndrome-related coronavirus ?
-
 ?
additional information the enzyme does not methylate m7G(5')pppG-RNA Severe acute respiratory syndrome-related coronavirus WHU ?
-
 ?
S-adenosyl-L-methionine + m7G(5')pppA-RNA
-
 Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + m7G(5')pppRA-RNA
-
 ?
S-adenosyl-L-methionine + m7G(5')pppA-RNA
-
 Severe acute respiratory syndrome-related coronavirus WHU S-adenosyl-L-homocysteine + m7G(5')pppRA-RNA
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Severe acute respiratory syndrome-related coronavirus

Synonyms

Synonyms Comment Organism
2'-O-MTase
-
 Severe acute respiratory syndrome-related coronavirus
2'O-MTase
-
 Severe acute respiratory syndrome-related coronavirus
nsp16
-
 Severe acute respiratory syndrome-related coronavirus
nsp16/nsp10 protein complex
-
 Severe acute respiratory syndrome-related coronavirus