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Literature summary for 2.1.1.57 extracted from
- Characterization of hMTr1, a human Cap1 2-O’-ribose methyltransferase (2010), J. Biol. Chem., 285, 33037-33044.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K239A | the mutant shows no activity | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | almost complete inhibition at 100 mM | Homo sapiens |  |
| S-adenosyl-L-homocysteine | inhibitory at concentrations beyond 0.002 mM | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the enzyme exhibits optimum activity in the presence of 2 mM MgCl2 | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-nitrilotriacetic acid resin column chromatography and heparin-Sepharose 6 column chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme catalyzes specific methylation of the 2'-O-ribose of the first nucleotide of a capped RNA transcript | Homo sapiens | ? | - |
? | |
| S-adenosyl-L-methionine + m7G(5')pppG-RNA | cap1 formation is optimal at 0.005 mM S-adenosyl-L-methionine | Homo sapiens | S-adenosyl-L-homocysteine + m7G(5')pppGm-RNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cap1 2-O'-ribose methyltransferase | - |
Homo sapiens |
| FtsJ methyltransferase domain-containing 2 | - |
Homo sapiens |
| FTSJD2 | - |
Homo sapiens |
| ISG95 | - |
Homo sapiens |
| Mtr1 | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
about pH 7.0 | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is responsible for cap1 formation in vivo | Homo sapiens |
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Release 2023.1 (January 2023)
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