Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2',3',5'-tri-O-acetyladenosine | about 20% increased activity at 0.1 mM | Dengue virus | |
2',3',5'-tri-O-acetyladenosine | about 50% increased activity at 0.1 mM | feline coronavirus | |
3-deazaadenosine | about 10% increased activity at 0.1 mM | feline coronavirus | |
5'-deoxy-5'-(methylthio)adenosine | about 75% increased activity at 0.1 mM | feline coronavirus | |
5'-deoxy-5'-methylthioadenosine | about 10% increased activity at 0.1 mM | Dengue virus | |
5'-S-isobutylthio-5'-deoxyadenosine | about 60% increased activity at 0.1 mM | feline coronavirus | |
ribavirin | about 10% increased activity at 0.1 mM | feline coronavirus | |
ribavirin triphosphate | about 20% increased activity at 0.1 mM | feline coronavirus |
Cloned (Comment) | Organism |
---|---|
- |
Dengue virus |
expressed in Escherichia coli C41(DE3) cells | feline coronavirus |
Protein Variants | Comment | Organism |
---|---|---|
D113A | about 110% substrate binding and activity compared to the wild type enzyme | feline coronavirus |
D129A | about 40% substrate binding and activity compared to the wild type enzyme | feline coronavirus |
D221A | about 115% substrate binding and activity compared to the wild type enzyme | feline coronavirus |
D247A | about 75% substrate binding and activity compared to the wild type enzyme | feline coronavirus |
E202A | about 30% substrate binding and activity compared to the wild type enzyme | feline coronavirus |
F173A | about 20% substrate binding and activity compared to the wild type enzyme | feline coronavirus |
K169A | about 20% substrate binding and activity compared to the wild type enzyme | feline coronavirus |
K45A | about 1% substrate binding and activity compared to the wild type enzyme | feline coronavirus |
W4A | about 90% substrate binding and activity compared to the wild type enzyme | feline coronavirus |
Y14A | about 10% substrate binding and activity compared to the wild type enzyme | feline coronavirus |
Y29A | about 15% substrate binding and activity compared to the wild type enzyme | feline coronavirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-S-isobutylthio-5'-deoxyadenosine | about 25% inhibition at 0.1 mM | Dengue virus | |
5-ethynyl-1-beta-D-ribofuranosylimidazole-4-carboxamide triphosphate | about 40% inhibition at 0.1 mM | Dengue virus | |
deazaadenosine | about 5% inhibition at 0.1 mM | Dengue virus | |
ribavirin | about 10% inhibition at 0.1 mM | Dengue virus | |
ribavirin triphosphate | about 50% inhibition at 0.1 mM | Dengue virus | |
S-(5'-adenosyl)-L-cysteine | about 25% inhibition at 0.1 mM | feline coronavirus | |
S-5'-adenosyl-L-cysteine | about 5% inhibition at 0.1 mM | Dengue virus | |
S-adenosyl-L-homocysteine | complete inhibition at 0.1 mM | Dengue virus | |
S-adenosyl-L-homocysteine | about 25% inhibition at 0.1 mM | feline coronavirus | |
sinefungin | complete inhibition at 0.1 mM | Dengue virus | |
sinefungin | about 5% inhibition at 0.1 mM | feline coronavirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000394 | - |
7Me-GpppA-C5 | in 40 mM Tris-HCl, pH 7.5, at 30°C | Dengue virus | |
0.00326 | - |
S-adenosyl-L-methionine | in 40 mM Tris-HCl, pH 7.5, at 30°C | Dengue virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 110% activity in the presence of 10 mM KCl, in Tris-HCl buffer (pH 7.5) | Dengue virus | |
Mg2+ | maximum activity in the presence of 1 mM | feline coronavirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | feline coronavirus |
65000 | - |
gel filtration | feline coronavirus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Dengue virus | maximum 2'O-MTase activity requires the cap and at least 6 nucleotides | ? | - |
? | |
additional information | feline coronavirus | the enzyme shows highest activity towards 7Me-GpppA-Cn where n equals at least four, and does not methylate GpppAC5 or pppAC5 | ? | - |
? | |
S-adenosyl-L-methionine + 7Me-GpppA-C5 | feline coronavirus | - |
S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-C5 | - |
? | |
S-adenosyl-L-methionine + 7Me-GpppA-C5 | Dengue virus | exclusive methylation at the 2'O position | S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-C5 | - |
? | |
S-adenosyl-L-methionine + 7Me-GpppA-Cn | Dengue virus | exclusive methylation at the 2'O position | S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-Cn | - |
? | |
S-adenosyl-L-methionine + GpppA-Cn | Dengue virus | exclusive methylation at the 2'O position | S-adenosyl-L-homocysteine + GpppA-2'OMe-Cn | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dengue virus | - |
- |
- |
feline coronavirus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Dengue virus |
Sepharose resin column chromatography and Superdex 200 gel filtration | feline coronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | maximum 2'O-MTase activity requires the cap and at least 6 nucleotides | Dengue virus | ? | - |
? | |
additional information | the enzyme shows highest activity towards 7Me-GpppA-Cn where n equals at least four, and does not methylate GpppAC5 or pppAC5 | feline coronavirus | ? | - |
? | |
S-adenosyl-L-methionine + 7Me-GpppA-C5 | - |
feline coronavirus | S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-C5 | - |
? | |
S-adenosyl-L-methionine + 7Me-GpppA-C5 | exclusive methylation at the 2'O position | Dengue virus | S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-C5 | - |
? | |
S-adenosyl-L-methionine + 7Me-GpppA-Cn | exclusive methylation at the 2'O position | Dengue virus | S-adenosyl-L-homocysteine + 7Me-GpppA-2'OMe-Cn | - |
? | |
S-adenosyl-L-methionine + GpppA-Cn | exclusive methylation at the 2'O position | Dengue virus | S-adenosyl-L-homocysteine + GpppA-2'OMe-Cn | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 35000, SDS-PAGE | feline coronavirus |
Synonyms | Comment | Organism |
---|---|---|
(nucleoside-2'O)-methyltransferase | part of the NS5 protein | Dengue virus |
2'O-MTase | - |
Dengue virus |
2'O-MTase | - |
feline coronavirus |
nonstructural protein 16 | - |
feline coronavirus |
nonstructural protein 16 methyltransferase | - |
feline coronavirus |
nonstructural protein 5 methyltransferase | - |
Dengue virus |
NS5MTase | the protein contains mRNA cap (guanine N-7) methyltransferase and (nucleoside-2'O)-methyltransferase activities | Dengue virus |
nsp16 | - |
feline coronavirus |
RNA (nucleoside-2'O)-methyltransferase | - |
feline coronavirus |
S-adenosyl-L-methionine-dependent mRNA cap MTase | - |
feline coronavirus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
feline coronavirus |
8 | - |
- |
Dengue virus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Dengue virus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | - |
at basic pH, 2'O-MTase activity is practically non-existent and neither Mg2+ nor KCl is able to activate the methyl transfer at pH 10.0 | Dengue virus |
10 | - |
at basic pH, 2'O-MTase activity is practically non-existent and neither Mg2+ nor KCl is able to activate the methyl transfer at pH 10.0 | feline coronavirus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00034 | - |
in 40 mM Tris-HCl, pH 7.5, at 30°C | Dengue virus | S-adenosyl-L-homocysteine | |
0.00063 | - |
in 40 mM Tris-HCl, pH 7.5, at 30°C | Dengue virus | sinefungin | |
0.144 | - |
- |
feline coronavirus | S-adenosyl-L-homocysteine |