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Literature summary for 2.1.1.54 extracted from
- Characterization of deoxycytidylate methyltransferase in Xanthomonas oryzae infected with bacteriophage Xp12 (1978), Eur. J. Biochem., 87, 29-36.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | above 1 mM | Xanthomonas oryzae |  |
| Mg2+ | above 50 mM | Xanthomonas oryzae | |
| Zn2+ | above 1 mM | Xanthomonas oryzae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0313 | - |
dCMP | - |
Xanthomonas oryzae | |
| 0.0714 | - |
5,10-methylenetetrahydrofolate | - |
Xanthomonas oryzae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 25 mM, slight stimulation | Xanthomonas oryzae | |
| Cu2+ | below 1 mM, stimulation | Xanthomonas oryzae | |
| K+ | 150 mM, stimulation | Xanthomonas oryzae | |
| Mg2+ | stimulates below 25 mM | Xanthomonas oryzae | |
| Mn2+ | has a similar effect in stimulation but not as strong as Mg2+ | Xanthomonas oryzae | |
| additional information | among the buffers tested, 6-morpholinoethane sulfonate/Mg2+ is the best | Xanthomonas oryzae | |
| Zn2+ | below 1 mM, stimulation | Xanthomonas oryzae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Xanthomonas oryzae | - |
phage Xp12-infected | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phage Xp12-infected, partial | Xanthomonas oryzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.11 | - |
- |
Xanthomonas oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + CMP | - |
Xanthomonas oryzae | dihydrofolate + 5-methylcytidylate | - |
? | |
| 5,10-methylenetetrahydrofolate + CTP | low activity | Xanthomonas oryzae | dihydrofolate + 5-methylcytidine 5'-triphosphate | - |
? | |
| 5,10-methylenetetrahydrofolate + dCMP | - |
Xanthomonas oryzae | dihydrofolate + deoxy-5-methylcytidylate | - |
? | |
| 5,10-methylenetetrahydrofolate + dCTP | low activity | Xanthomonas oryzae | dihydrofolate + deoxy-5-methylcytidine 5'-triphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Xanthomonas oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
3 min, 90% loss of activity | Xanthomonas oryzae |
| 70 | - |
3 min, complete loss of activity | Xanthomonas oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Xanthomonas oryzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Xanthomonas oryzae |
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