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Literature summary for 2.1.1.50 extracted from

  • Murata, J.; Roepke, J.; Gordon, H.; De Luca, V.
    The leaf epidermome of Catharanthus roseus reveals its biochemical specialization (2008), Plant Cell, 20, 524-542.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.50

Application

Application Comment Organism
pharmacology Catharanthus roseus is the sole commercial source for monoterpenoid indole alkaloids vindoline and catharanthine, components of the anticancer dimers vinblastine and vincristine Catharanthus roseus

Cloned(Commentary)

Cloned (Comment) Organism
PCR-amplification of cDNA, insertion into expression vector pET 30b, ligated into pGEM-TEasy, heat shock transformation of Escherichia coli DE3-MRF Catharanthus roseus

Inhibitors

Inhibitors Comment Organism Structure
loganin reaction product Catharanthus roseus
S-adenosyl-L-homocysteine
-
 Catharanthus roseus
S-adenosyl-L-methionine
-
 Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
14.76
-
 loganic acid pH 7.5, 37°C Catharanthus roseus
742.1
-
 S-adenosyl-L-methionine pH 7.5, 37°C Catharanthus roseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42020
-
 calculated from 371-amino acid open reading frame Catharanthus roseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
loganic acid + S-adenosyl-L-methionine Catharanthus roseus
-
 loganin + S-adenosyl-L-homocysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Catharanthus roseus B2KPR3 cultivar Little Delicata, Madagaskar periwinkle
-

Purification (Commentary)

Purification (Comment) Organism
crude recombinant enzyme, for enzyme activity location study upper and lower epidermis of leaves is abraded with carborundum, leaves rubbed with cotton swabs and then dipped in Trizol at 4°C and agitated Catharanthus roseus

Source Tissue

Source Tissue Comment Organism Textmining
epidermis
-
 Catharanthus roseus
-
flower highest activity Catharanthus roseus
-
leaf 10fold higher enzyme activity in epidermis extracts than in whole leaves, 50% lower activity in aged L2, L3, and L4 leaves compared to young L1 leaves Catharanthus roseus
-
root 4-8times lower activity than in the other tested plant organs Catharanthus roseus
-
silique similar activity as in leaves Catharanthus roseus
-
stem second highest activity Catharanthus roseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000000517
-
 secologanic acid, buffer (100 mM Tris-HCl, pH 7.5, 14 mM beta-mercaptoethanol, 25 mM KCl), 37°C, 30 min assay Catharanthus roseus
0.00000517
-
 loganic acid, buffer (100 mM Tris-HCl, pH 7.5, 14 mM beta-mercaptoethanol, 25 mM KCl), 37°C, 30 min assay Catharanthus roseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
loganic acid + S-adenosyl-L-methionine
-
 Catharanthus roseus loganin + S-adenosyl-L-homocysteine
-
 ?
secologanic acid + S-adenosyl-L-methionine 10% activity compared to loganic acid Catharanthus roseus secologanin + S-adenosyl-L-homocysteine
-
 ?

Synonyms

Synonyms Comment Organism
LAMT
-
 Catharanthus roseus
loganic acid O-methyltransferase
-
 Catharanthus roseus
rLAMT recombinant LAMT Catharanthus roseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.327
-
 loganic acid pH 7.5, 37°C Catharanthus roseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Catharanthus roseus

Cofactor

Cofactor Comment Organism Structure
additional information Mg2+ or K+ are not required for enzyme activity nor to enhance it Catharanthus roseus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 S-adenosyl-L-methionine pH 7.5, 37°C Catharanthus roseus
0.215
-
 loganin pH 7.5, 37°C Catharanthus roseus
0.4
-
 S-adenosyl-L-homocysteine pH 7.5, 37°C Catharanthus roseus

General Information

General Information Comment Organism
metabolism enzyme in the monoterpenoid indole alkaloid biosynthesis pathway Catharanthus roseus