General Stability | Organism |
---|---|
glycerol does not stabilize | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Pinanyl N,N,N-trimethylaminomethane boronate | substrate analogue | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-homocysteine | betaine | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
6 * 45000, SDS-PAGE | Rattus norvegicus |
270000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus Sprague-Dawley | - |
- |
- |
Purification (Comment) | Organism |
---|---|
FPLC-chromatofocusing, 3 active forms | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0187 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, 2 weeks in the presence of substrates | Rattus norvegicus |
-20°C, 40% loss of activity after 4 weeks in the presence of substrates | Rattus norvegicus |
-20°C, 70% loss of activity after 10 weeks in the presence of substrates | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N,N-trimethylglycine + L-homocysteine | - |
Rattus norvegicus | N,N-dimethylglycine + L-methionine | - |
? | |
N,N,N-trimethylglycine + L-homocysteine | - |
Rattus norvegicus Sprague-Dawley | N,N-dimethylglycine + L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 45000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
3 active forms with pI: 7.0, 7.6 and 8.0 | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
Pinanyl N,N,N-trimethylaminomethane boronate | - |
Rattus norvegicus |