General Stability | Organism |
---|---|
freezing inactivates | Streptomyces griseus |
heating inactivates | Streptomyces griseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Streptomyces griseus | |
2,2'-bipyridyl | - |
Streptomyces griseus | |
iodoacetamide | weak | Streptomyces griseus | |
iodoacetic acid | weak | Streptomyces griseus | |
additional information | no inhibition by EDTA; not inhibitory: indolmycin, L-tryptophan, diethyldithiocarbamate, L-tryptophanylglycine, L-tryptophanamide | Streptomyces griseus | |
N-ethylmaleimide | - |
Streptomyces griseus | |
p-chloromercuribenzoate | strong, reversible by cysteine | Streptomyces griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
Indolepyruvate | - |
Streptomyces griseus | |
0.013 | - |
S-adenosyl-L-methionine | - |
Streptomyces griseus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for Mg2+, Mn2+, Ca2+ | Streptomyces griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Streptomyces griseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + indolepyruvate | Streptomyces griseus | reaction in indolmycin biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces griseus |
Storage Stability | Organism |
---|---|
2°C, up to 1 month | Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrate: L-tryptophan | Streptomyces griseus | ? | - |
? | |
additional information | no substrates are phenylpyruvate, p-hydroxyphenylpyruvate | Streptomyces griseus | ? | - |
? | |
S-adenosyl-L-methionine + indolepyruvate | - |
Streptomyces griseus | S-adenosyl-L-homocysteine + (S)-beta-methylindolepyruvate | - |
? | |
S-adenosyl-L-methionine + indolepyruvate | reaction in indolmycin biosynthesis | Streptomyces griseus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
heating inactivates | Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
phosphate buffer | Streptomyces griseus |
7.5 | 8.5 | - |
Streptomyces griseus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
and below, irreversible inactivation | Streptomyces griseus |