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Literature summary for 2.1.1.47 extracted from

  • Speedie, M.K.; Hornemann, U.; Floss, H.G.
    Isolation and characterization of tryptophan transaminase and indolepyruvate C-methyltransferase. Enzymes involved in indolmycin biosynthesis in Streptomyces griseus (1975), J. Biol. Chem., 250, 7819-7825.
    View publication on PubMed

General Stability

General Stability Organism
freezing inactivates Streptomyces griseus
heating inactivates Streptomyces griseus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Streptomyces griseus
2,2'-bipyridyl
-
Streptomyces griseus
iodoacetamide weak Streptomyces griseus
iodoacetic acid weak Streptomyces griseus
additional information no inhibition by EDTA; not inhibitory: indolmycin, L-tryptophan, diethyldithiocarbamate, L-tryptophanylglycine, L-tryptophanamide Streptomyces griseus
N-ethylmaleimide
-
Streptomyces griseus
p-chloromercuribenzoate strong, reversible by cysteine Streptomyces griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0048
-
Indolepyruvate
-
Streptomyces griseus
0.013
-
S-adenosyl-L-methionine
-
Streptomyces griseus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for Mg2+, Mn2+, Ca2+ Streptomyces griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
gel filtration Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + indolepyruvate Streptomyces griseus reaction in indolmycin biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces griseus

Storage Stability

Storage Stability Organism
2°C, up to 1 month Streptomyces griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: L-tryptophan Streptomyces griseus ?
-
?
additional information no substrates are phenylpyruvate, p-hydroxyphenylpyruvate Streptomyces griseus ?
-
?
S-adenosyl-L-methionine + indolepyruvate
-
Streptomyces griseus S-adenosyl-L-homocysteine + (S)-beta-methylindolepyruvate
-
?
S-adenosyl-L-methionine + indolepyruvate reaction in indolmycin biosynthesis Streptomyces griseus ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
heating inactivates Streptomyces griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
phosphate buffer Streptomyces griseus
7.5 8.5
-
Streptomyces griseus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
and below, irreversible inactivation Streptomyces griseus