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Literature summary for 2.1.1.45 extracted from
- Biochemical characterization of two thymidylate synthases in Corynebacterium glutamicum NCHU 87078 (2010), Biochim. Biophys. Acta, 1804, 1751-1759.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli NovaBlue cells | Corynebacterium glutamicum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29100 | - |
x * 29100, SDS-PAGE | Corynebacterium glutamicum |
| 29400 | - |
x * 29400, calculated from amino acid sequence | Corynebacterium glutamicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | - |
- |
- |
| Corynebacterium glutamicum NCHU 87078 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-chelate column chromatography, gel filtration | Corynebacterium glutamicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 414 | - |
purified enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 37°C | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | - |
Corynebacterium glutamicum | dihydrofolate + dTMP | - |
? |  |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Corynebacterium glutamicum NCHU 87078 | dihydrofolate + dTMP | - |
? | |
| additional information | thymidylate synthase form ThyX has no activity in Corynebacterium glutamicum | Corynebacterium glutamicum | ? | - |
? | |
| additional information | thymidylate synthase form ThyX has no activity in Corynebacterium glutamicum | Corynebacterium glutamicum NCHU 87078 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29100, SDS-PAGE | Corynebacterium glutamicum |
| ? | x * 29400, calculated from amino acid sequence | Corynebacterium glutamicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ThyA | - |
Corynebacterium glutamicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is FAD-independent | Corynebacterium glutamicum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | ThyA is the sole catalyst for thymidylate biosynthesis in Corynebacterium glutamicum | Corynebacterium glutamicum |
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