Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain TX61- cells and in the RJK88.13 cell line (an enzyme-deficient derivative of V79 Chinese hamster lung cells) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
V3L, V3F and V3Y mutant enzymes, hanging drop vapor diffusion method, using 100 mM Tris-base, pH 8.5, 20 mM 2-mercaptoethanol, 32-40% (w/v) ammonium sulfate and 16-24% (w/v) polyethylene glycol 4000 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
V3A | the mutant enzyme has an intracellular half-life of approximately 6 h after treatment with cycloheximide | Homo sapiens |
V3F | the mutant enzyme has an intracellular half-life of approximately 3.5 h after treatment with cycloheximide | Homo sapiens |
V3L | the mutant enzyme has an intracellular half-life of approximately 2.5 h after treatment with cycloheximide | Homo sapiens |
V3T | the mutant enzyme has an intracellular half-life of approximately 24 h after treatment with cycloheximide | Homo sapiens |
V3Y | the mutant enzyme has an intracellular half-life of approximately 2.5 h after treatment with cycloheximide | Homo sapiens |
General Stability | Organism |
---|---|
the wild type enzyme has an intracellular half-life of approximately 6 h after treatment with cycloheximide | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
dUMP | apparent value, wild type enzyme, pH not specified in the publication, at 37°C | Homo sapiens | |
0.0044 | - |
dUMP | apparent value, mutant enzyme V3Y, pH not specified in the publication, at 37°C | Homo sapiens | |
0.011 | - |
dUMP | apparent value, mutant enzyme V3T, pH not specified in the publication, at 37°C | Homo sapiens | |
0.022 | - |
dUMP | apparent value, mutant enzyme V3A, pH not specified in the publication, at 37°C | Homo sapiens | |
0.109 | - |
dUMP | apparent value, mutant enzyme V3L, pH not specified in the publication, at 37°C | Homo sapiens | |
0.134 | - |
dUMP | apparent value, mutant enzyme V3F, pH not specified in the publication, at 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Homo sapiens |