Literature summary for 2.1.1.45 extracted from

Arvizu-Flores, A.A.; Aispuro-Hernandez, E.; Garcia-Orozco, K.D.; Varela-Romero, A.; Valenzuela-Soto, E.; Velazquez-Contreras, E.F.; Rojo-Dominguez, A.; Yepiz-Plascencia, G.; Maley, F.; Sotelo-Mundo, R.R.
Functional identity of the active sites of crustacean and viral thymidylate synthases (2009), Comp. Biochem. Physiol. C, 150, 406-413.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Shrimp white spot syndrome virus
expressed in Escherichia coli BL21(DE3) cells	Penaeus vannamei

Inhibitors

Inhibitors	Comment	Organism	Structure
(S)-2-(5(((1,2-dihydro-3-methyl-1-oxobenzo[f]quinazolin-9 yl)methyl)amino)1-oxo-2-isoindolinyl)glutaric acid	-	Penaeus vannamei
(S)-2-(5(((1,2-dihydro-3-methyl-1-oxobenzo[f]quinazolin-9-yl)methyl)amino)1-oxo-2-isoindolinyl)glutaric acid	-	Shrimp white spot syndrome virus
10-propargyl-5,8-dideazafolate	-	Penaeus vannamei
10-propargyl-5,8-dideazafolate	-	Shrimp white spot syndrome virus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0012	-	dUMP	at 30°C	Shrimp white spot syndrome virus
0.0021	-	dUMP	at 30°C	Penaeus vannamei
0.0134	-	5,10-methylenetetrahydrofolate	at 30°C	Shrimp white spot syndrome virus
0.0135	-	5,10-methylenetetrahydrofolate	at 30°C	Penaeus vannamei

Organism

Organism	UniProt	Comment	Textmining
Penaeus vannamei	C6GJB8	-	-
Shrimp white spot syndrome virus	Q8QTF3	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and Q-Sepharose column chromatography	Shrimp white spot syndrome virus
ammonium sulfate precipitation and Q-Sepharose column chromatography	Penaeus vannamei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,10-methylenetetrahydrofolate + dUMP	-	Shrimp white spot syndrome virus	dihydrofolate + dTMP	-	?
5,10-methylenetetrahydrofolate + dUMP	-	Penaeus vannamei	dihydrofolate + dTMP	-	?

Synonyms

Synonyms	Comment	Organism
ThyA	-	Shrimp white spot syndrome virus
ThyA	-	Penaeus vannamei

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.8	-	5,10-methylenetetrahydrofolate	at 30°C	Shrimp white spot syndrome virus
4.1	-	5,10-methylenetetrahydrofolate	at 30°C	Penaeus vannamei

Cofactor

Cofactor	Comment	Organism	Structure
N5,N10-methylenetetrahydrofolate	-	Shrimp white spot syndrome virus
N5,N10-methylenetetrahydrofolate	-	Penaeus vannamei

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00015	-	at 30°C	Penaeus vannamei	(S)-2-(5(((1,2-dihydro-3-methyl-1-oxobenzo[f]quinazolin-9 yl)methyl)amino)1-oxo-2-isoindolinyl)glutaric acid
0.00017	-	at 30°C	Penaeus vannamei	10-propargyl-5,8-dideazafolate
0.00019	-	at 30°C	Shrimp white spot syndrome virus	(S)-2-(5(((1,2-dihydro-3-methyl-1-oxobenzo[f]quinazolin-9 yl)methyl)amino)1-oxo-2-isoindolinyl)glutaric acid
0.00023	-	at 30°C	Shrimp white spot syndrome virus	10-propargyl-5,8-dideazafolate

General Information

General Information	Comment	Organism
physiological function	thymidylate synthase is the only de novo source of dTMP and is essential for DNA replication	Shrimp white spot syndrome virus
physiological function	thymidylate synthase is the only de novo source of dTMP and is essential for DNA replication	Penaeus vannamei

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Release 2023.1 (January 2023)