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Literature summary for 2.1.1.45 extracted from
- Kinetics and ligand-binding preferences of Mycobacterium tuberculosis thymidylate synthases, ThyA and ThyX (2008), PLoS ONE, 3, e2237.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | thymidylate synthase as a target for antitubercular drugs | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mycobacterium tuberculosis |
| histidine-tagged Mycobacterium tuberculosis ThyA is overexpressed from BL21-DE3 pLysS Escherichia coli | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1843U89 | i.e. (2R)-2-(5-[[(1-hydroxy-3-methyl-5,10a-dihydrobenzo[f]quinazolin-9-yl)methyl]amino]-1-oxo-1,3-dihydro-2H-isoindol-2-yl)butanedioic acid, folate-based inhibitor | Mycobacterium tuberculosis |  |
| 5-fluoro-2'-deoxyuridine 5'-monophosphate | FdUMP | Mycobacterium tuberculosis | |
| GW1843U89 | - |
Mycobacterium tuberculosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
dUMP | 22°C | Mycobacterium tuberculosis | |
| 0.004 | - |
dUMP | - |
Mycobacterium tuberculosis | |
| 0.004 | - |
5,10-methylenetetrahydrofolate | 22°C | Mycobacterium tuberculosis | |
| 0.004 | - |
dUMP | 22°C | Mycobacterium tuberculosis | |
| 0.07 | - |
5,10-methylenetetrahydrofolate | - |
Mycobacterium tuberculosis | |
| 0.07 | - |
5,10-methylenetetrahydrofolate | 22°C | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28700 | - |
4 * 28700, calculated | Mycobacterium tuberculosis |
| 30700 | - |
2 * 30700, calculated | Mycobacterium tuberculosis |
| 57000 | - |
gel filtration | Mycobacterium tuberculosis |
| 104000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | Mycobacterium tuberculosis | - |
7,8-dihydrofolate + dTMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis | - |
isoform ThyX | - |
| Mycobacterium tuberculosis | P9WFR9 | isoform ThyA | - |
| Mycobacterium tuberculosis H37Rv | P9WFR9 | isoform ThyA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel affinity, ionexchange, and gel filtration | Mycobacterium tuberculosis |
| recombinant protein | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 210 | - |
22°C, recombinant protein | Mycobacterium tuberculosis |
| 210 | - |
22°C, recombinant protien | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mycobacterium tuberculosis | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mycobacterium tuberculosis H37Rv | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mycobacterium tuberculosis | 7,8-dihydrofolate + dTMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30700, calculated | Mycobacterium tuberculosis |
| dimer | predicted mass of a dimer | Mycobacterium tuberculosis |
| tetramer | 4 * 28700, calculated | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ThyA | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
dUMP | 22°C | Mycobacterium tuberculosis | |
| 18 | - |
dUMP | 22°C | Mycobacterium tuberculosis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000002 | - |
5-fluoro-2'-deoxyuridine 5'-monophosphate | 22°C | Mycobacterium tuberculosis | |
| 0.000002 | - |
5-fluoro-2'-deoxyuridine 5'-monophosphate | inhibited both ThyA and ThyX | Mycobacterium tuberculosis | |
| 0.000018 | - |
GW1843U89 | - |
Mycobacterium tuberculosis | |
| 0.000018 | - |
(2R)-2-(5-[[(1-hydroxy-3-methyl-5,10a-dihydrobenzo[f]quinazolin-9-yl)methyl]amino]-1-oxo-1,3-dihydro-2H-isoindol-2-yl)butanedioic acid | 22°C | Mycobacterium tuberculosis | |
| 0.0001 | - |
5-fluoro-2'-deoxyuridine 5'-monophosphate | 22°C | Mycobacterium tuberculosis | |
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