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Literature summary for 2.1.1.45 extracted from
- 5-Substituted N4-hydroxy-2'-deoxycytidines and their 5'-monophosphates: Synthesis, conformation, interaction with tumor thymidylate synthase, and in vitro antitumor activity (2000), J. Med. Chem., 43, 4647-4656.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-bromopyrimidin-2(1H)-one 5'-monophosphate | - |
Mus musculus |  |
| 1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-chloropyrimidin-2(1H)-one 5'-monophosphate | - |
Mus musculus | |
| 1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-fluoropyrimidin-2(1H)-one 5'-monophosphate | - |
Mus musculus | |
| 1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-hydroxymethylpyrimidin-2(1H)-one 5'-monophosphate | - |
Mus musculus | |
| 1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-iodopyrimidin-2(1H)-one 5'-monophosphate | - |
Mus musculus | |
| 1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyaminopyrimidin-2(1H)-one 5'-monophosphate | - |
Mus musculus | |
| 5-bromo-N4-hydroxy-2'-deoxycytidine | - |
Mus musculus | |
| 5-chloro-N4-hydroxy-2'-deoxycytidine | - |
Mus musculus | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus | |
| bromo-2'-deoxyuridine | - |
Mus musculus | |
| chloro-2'-deoxyuridine | - |
Mus musculus | |
| hydroxymethyl-2'-deoxyuridine | - |
Mus musculus | |
| iodo-2'-deoxyuridine | - |
Mus musculus | |
| N4-hydroxy-2'-deoxycytidine | - |
Mus musculus | |
| N4-hydroxy-5-methyl-2'-deoxycytidine | - |
Mus musculus | |
| thymidine phosphate | - |
Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Rattus norvegicus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Trichinella spiralis | - |
dihydrofolate + dTMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lacticaseibacillus casei | - |
- |
- |
| Mus musculus | - |
L5178 murine leukemia cell line | - |
| Rattus norvegicus | - |
- |
- |
| Trichinella spiralis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.6 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Rattus norvegicus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Rattus norvegicus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trichinella spiralis | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Trichinella spiralis | dihydrofolate + dTMP | - |
r | |
| 5-bromo-2'-deoxyuridine 5'-monophosphate + ? | dehalogenation | Mus musculus | ? | - |
? | |
| 5-bromo-2'-deoxyuridine 5'-monophosphate + ? | dehalogenation | Lacticaseibacillus casei | ? | - |
? | |
| 5-bromo-2'-deoxyuridine 5'-monophosphate + ? | dehalogenated by recombinant rat hepatoma enzyme | Rattus norvegicus | ? | - |
? | |
| 5-iodo-2'-deoxyuridine 5'-monophosphate + ? | dehalogenation | Mus musculus | ? | - |
? | |
| 5-iodo-2'-deoxyuridine 5'-monophosphate + ? | dehalogenated by recombinant rat hepatoma enzyme | Rattus norvegicus | ? | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| N5,N10-methylenetetrahydrofolate | - |
Mus musculus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00002 | - |
5-fluoro-2'-deoxyuridylic acid | L1210 parental enzyme | Mus musculus | |
| 0.0003 | - |
chloro-2'-deoxyuridine | L1210 parental enzyme | Mus musculus | |
| 0.0008 | - |
1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-fluoropyrimidin-2(1H)-one 5'-monophosphate | L1210 parental enzyme | Mus musculus | |
| 0.0013 | - |
1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-hydroxymethylpyrimidin-2(1H)-one 5'-monophosphate | L1210 recombinant enzyme | Mus musculus | |
| 0.0014 | - |
1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-fluoropyrimidin-2(1H)-one 5'-monophosphate | L1210 recombinant enzyme | Mus musculus | |
| 0.0015 | - |
bromo-2'-deoxyuridine | L1210 parental enzyme | Mus musculus | |
| 0.0017 | - |
1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyaminopyrimidin-2(1H)-one 5'-monophosphate | L1210 parental enzyme | Mus musculus | |
| 0.00191 | - |
1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-hydroxymethylpyrimidin-2(1H)-one 5'-monophosphate | L1210 parental enzyme | Mus musculus | |
| 0.0034 | - |
1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-iodopyrimidin-2(1H)-one 5'-monophosphate | L1210 recombinant enzyme | Mus musculus | |
| 0.0037 | - |
hydroxymethyl-2'-deoxyuridine | L1210 parental enzyme | Mus musculus | |
| 0.0049 | - |
1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-chloropyrimidin-2(1H)-one 5'-monophosphate | L1210 recombinant enzyme | Mus musculus | |
| 0.0073 | - |
thymidine phosphate | L1210 parental enzyme | Mus musculus | |
| 0.00746 | - |
1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-iodopyrimidin-2(1H)-one 5'-monophosphate | L1210 parental enzyme | Mus musculus | |
| 0.00768 | - |
iodo-2'-deoxyuridine | L1210 parental enzyme | Mus musculus | |
| 0.0085 | - |
1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-chloropyrimidin-2(1H)-one 5'-monophosphate | L1210 parental enzyme | Mus musculus | |
| 0.0093 | - |
1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyaminopyrimidin-2(1H)-one 5'-monophosphate | L1210 recombinant enzyme | Mus musculus | |
| 0.022 | - |
1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-bromopyrimidin-2(1H)-one 5'-monophosphate | L1210 recombinant enzyme | Mus musculus | |
| 0.0321 | - |
1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-bromopyrimidin-2(1H)-one 5'-monophosphate | L1210 parental enzyme | Mus musculus | |
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