Application | Comment | Organism |
---|---|---|
medicine | - |
Mus musculus |
medicine | - |
Homo sapiens |
medicine | - |
Crithidia fasciculata |
medicine | - |
Plasmodium falciparum |
medicine | development of effective chemotherapy of the eosinophilic meningoencephalitis angiostrongylosis | Angiostrongylus cantonensis |
Cloned (Comment) | Organism |
---|---|
- |
Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-2'-deoxyuridylic acid | - |
Angiostrongylus cantonensis | |
5-fluoro-2'-deoxyuridylic acid | - |
Homo sapiens | |
5-fluoro-2'-deoxyuridylic acid | - |
Leishmania major | |
5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus | |
5-fluoro-2'-deoxyuridylic acid | - |
Plasmodium falciparum | |
5-fluoro-2'-deoxyuridylic acid | - |
Rattus norvegicus | |
5-fluoro-2'-deoxyuridylic acid | - |
Saccharomyces cerevisiae | |
aminofolate | - |
Angiostrongylus cantonensis | |
dihydrofolate | - |
Angiostrongylus cantonensis | |
methotrexate | - |
Angiostrongylus cantonensis | |
methotrexate | - |
Homo sapiens | |
methotrexate | - |
Saccharomyces cerevisiae | |
thymidine phosphate | - |
Angiostrongylus cantonensis | |
thymidine phosphate | - |
Bos taurus | |
thymidine phosphate | - |
Mus musculus | |
trimethoprim | - |
Angiostrongylus cantonensis | |
trimethoprim | - |
Crithidia fasciculata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
2'-deoxyuridylate | - |
Angiostrongylus cantonensis | |
0.185 | - |
5,10-methylenetetrahydrofolate | - |
Angiostrongylus cantonensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
2 * 31000, SDS-PAGE | Angiostrongylus cantonensis |
66000 | - |
gel filtration | Homo sapiens |
66000 | - |
gel filtration | Angiostrongylus cantonensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Gallus gallus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Rattus norvegicus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Sus scrofa | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Saccharomyces cerevisiae | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Bos taurus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Crithidia fasciculata | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Aedes aegypti | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Candida albicans | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Plasmodium falciparum | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Leishmania major | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Brugia pahangi | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Toxoplasma gondii | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Mus musculus | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Angiostrongylus cantonensis | key function in DNA synthesis, repair and cell division | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Angiostrongylus cantonensis | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aedes aegypti | - |
- |
- |
Angiostrongylus cantonensis | - |
metastrongyloid nematode, synonym Parastrongylus | - |
Bos taurus | - |
calf | - |
Brugia pahangi | - |
- |
- |
Candida albicans | - |
- |
- |
Crithidia fasciculata | - |
- |
- |
Gallus gallus | - |
chicken | - |
Homo sapiens | - |
human | - |
Leishmania major | - |
- |
- |
Mus musculus | - |
Ehrlich ascites carcinoma cells | - |
Plasmodium falciparum | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Sus scrofa | - |
pig | - |
Toxoplasma gondii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
- |
Homo sapiens |
- |
Bos taurus |
- |
Candida albicans |
- |
Plasmodium falciparum |
- |
Angiostrongylus cantonensis |
recombinant enzyme | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blast cell | - |
Homo sapiens | - |
fetus | - |
Rattus norvegicus | - |
fetus | - |
Sus scrofa | - |
fibroblast | - |
Mus musculus | - |
heart | parasitic helminth, obtained from the pulmonary hearts and vessels of infected Wistar albino rats | Angiostrongylus cantonensis | - |
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
thymus | - |
Mus musculus | - |
thymus | - |
Homo sapiens | - |
thymus | - |
Sus scrofa | - |
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0003367 | - |
- |
Angiostrongylus cantonensis |
Storage Stability | Organism |
---|---|
-70°C preparation with 0.1% bovine serum albumin is stable up to 8 weeks of storage | Angiostrongylus cantonensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Gallus gallus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Gallus gallus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Rattus norvegicus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Rattus norvegicus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Sus scrofa | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Sus scrofa | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Crithidia fasciculata | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Crithidia fasciculata | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Aedes aegypti | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Aedes aegypti | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Candida albicans | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Candida albicans | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Plasmodium falciparum | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Plasmodium falciparum | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Leishmania major | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Leishmania major | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Brugia pahangi | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Brugia pahangi | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Angiostrongylus cantonensis | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Toxoplasma gondii | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Toxoplasma gondii | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Mus musculus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Homo sapiens | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | key function in DNA synthesis, repair and cell division | Angiostrongylus cantonensis | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Homo sapiens | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Angiostrongylus cantonensis | dihydrofolate + dTMP | - |
? | |
additional information | - |
Homo sapiens | ? | - |
? | |
additional information | bifunctional enzyme dihydrofolate reductase-thymidylate synthase | Plasmodium falciparum | ? | - |
? | |
additional information | bifunctional enzyme dihydrofolate reductase-thymidylate synthase | Leishmania major | ? | - |
? | |
additional information | bifunctional enzyme dihydrofolate reductase-thymidylate synthase | Toxoplasma gondii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Mus musculus |
dimer | - |
Homo sapiens |
dimer | - |
Rattus norvegicus |
dimer | - |
Saccharomyces cerevisiae |
dimer | - |
Bos taurus |
dimer | - |
Plasmodium falciparum |
dimer | - |
Leishmania major |
dimer | 2 * 31000, SDS-PAGE | Angiostrongylus cantonensis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000021 | - |
5-fluoro-2'-deoxyuridylic acid | 50 nM | Angiostrongylus cantonensis | |
0.0000235 | - |
5-fluoro-2'-deoxyuridylic acid | - |
Angiostrongylus cantonensis | |
0.000026 | - |
5-fluoro-2'-deoxyuridylic acid | 200 nM | Angiostrongylus cantonensis | |
0.000833 | - |
trimethoprim | 1.0 mM | Angiostrongylus cantonensis | |
0.00087 | - |
trimethoprim | 2.0 mM | Angiostrongylus cantonensis | |
0.001 | - |
thymidine phosphate | 0.25 mM, noncompetitive inhibition, 5,10-mehylenetetrahydrofolate as variable substrate | Angiostrongylus cantonensis | |
0.00124 | - |
thymidine phosphate | 1.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate | Angiostrongylus cantonensis | |
0.0014 | - |
dihydrofolate | 1.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate | Angiostrongylus cantonensis | |
0.00178 | - |
dihydrofolate | 5.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate | Angiostrongylus cantonensis | |
0.00294 | - |
dihydrofolate | 0.5 mM, noncompetitive inhibition, dUMP as variable substrate | Angiostrongylus cantonensis | |
0.00303 | - |
dihydrofolate | 1.0 mM, noncompetitive inhibition, dUMP as variable substrate | Angiostrongylus cantonensis | |
0.238 | - |
methotrexate | 0.05 mM | Angiostrongylus cantonensis | |
0.267 | - |
methotrexate | 0.02 mM | Angiostrongylus cantonensis | |
0.3125 | - |
thymidine phosphate | 0.25 mM, competitive inhibition, dUMP as variable substrate | Angiostrongylus cantonensis | |
0.333 | - |
thymidine phosphate | - |
Angiostrongylus cantonensis | |
0.406 | - |
methotrexate | 0.5 mM | Angiostrongylus cantonensis | |
0.413 | - |
methotrexate | 1.0 mM | Angiostrongylus cantonensis | |
0.852 | - |
trimethoprim | - |
Angiostrongylus cantonensis | |
5 | - |
aminofolate | 1.0 mM | Angiostrongylus cantonensis | |
5.2 | - |
aminofolate | 5.0 mM | Angiostrongylus cantonensis |