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Literature summary for 2.1.1.45 extracted from

  • So, N.N.C.; Wong, P.C.L.; Ko, R.C.
    Angiostrongylus cantonensis: Characterization of thymidylate synthetase (1994), Exp. Parasitol., 79, 526-535.
    View publication on PubMed

Application

Application Comment Organism
medicine
-
Mus musculus
medicine
-
Homo sapiens
medicine
-
Crithidia fasciculata
medicine
-
Plasmodium falciparum
medicine development of effective chemotherapy of the eosinophilic meningoencephalitis angiostrongylosis Angiostrongylus cantonensis

Cloned(Commentary)

Cloned (Comment) Organism
-
Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
5-fluoro-2'-deoxyuridylic acid
-
Angiostrongylus cantonensis
5-fluoro-2'-deoxyuridylic acid
-
Homo sapiens
5-fluoro-2'-deoxyuridylic acid
-
Leishmania major
5-fluoro-2'-deoxyuridylic acid
-
Mus musculus
5-fluoro-2'-deoxyuridylic acid
-
Plasmodium falciparum
5-fluoro-2'-deoxyuridylic acid
-
Rattus norvegicus
5-fluoro-2'-deoxyuridylic acid
-
Saccharomyces cerevisiae
aminofolate
-
Angiostrongylus cantonensis
dihydrofolate
-
Angiostrongylus cantonensis
methotrexate
-
Angiostrongylus cantonensis
methotrexate
-
Homo sapiens
methotrexate
-
Saccharomyces cerevisiae
thymidine phosphate
-
Angiostrongylus cantonensis
thymidine phosphate
-
Bos taurus
thymidine phosphate
-
Mus musculus
trimethoprim
-
Angiostrongylus cantonensis
trimethoprim
-
Crithidia fasciculata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
2'-deoxyuridylate
-
Angiostrongylus cantonensis
0.185
-
5,10-methylenetetrahydrofolate
-
Angiostrongylus cantonensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
2 * 31000, SDS-PAGE Angiostrongylus cantonensis
66000
-
gel filtration Homo sapiens
66000
-
gel filtration Angiostrongylus cantonensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP Gallus gallus
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Rattus norvegicus
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Sus scrofa
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Saccharomyces cerevisiae
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Bos taurus
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Crithidia fasciculata
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Aedes aegypti
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Candida albicans
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Plasmodium falciparum
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Leishmania major
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Brugia pahangi
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Toxoplasma gondii
-
dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Mus musculus key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Homo sapiens key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Angiostrongylus cantonensis key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Homo sapiens biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP Angiostrongylus cantonensis biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?

Organism

Organism UniProt Comment Textmining
Aedes aegypti
-
-
-
Angiostrongylus cantonensis
-
metastrongyloid nematode, synonym Parastrongylus
-
Bos taurus
-
calf
-
Brugia pahangi
-
-
-
Candida albicans
-
-
-
Crithidia fasciculata
-
-
-
Gallus gallus
-
chicken
-
Homo sapiens
-
human
-
Leishmania major
-
-
-
Mus musculus
-
Ehrlich ascites carcinoma cells
-
Plasmodium falciparum
-
-
-
Rattus norvegicus
-
-
-
Saccharomyces cerevisiae
-
-
-
Sus scrofa
-
pig
-
Toxoplasma gondii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus gallus
-
Homo sapiens
-
Bos taurus
-
Candida albicans
-
Plasmodium falciparum
-
Angiostrongylus cantonensis
recombinant enzyme Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
blast cell
-
Homo sapiens
-
fetus
-
Rattus norvegicus
-
fetus
-
Sus scrofa
-
fibroblast
-
Mus musculus
-
heart parasitic helminth, obtained from the pulmonary hearts and vessels of infected Wistar albino rats Angiostrongylus cantonensis
-
liver
-
Mus musculus
-
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-
liver
-
Sus scrofa
-
thymus
-
Mus musculus
-
thymus
-
Homo sapiens
-
thymus
-
Sus scrofa
-
thymus
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0003367
-
-
Angiostrongylus cantonensis

Storage Stability

Storage Stability Organism
-70°C preparation with 0.1% bovine serum albumin is stable up to 8 weeks of storage Angiostrongylus cantonensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP
-
Gallus gallus dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Gallus gallus dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Mus musculus dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Homo sapiens dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Rattus norvegicus dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Rattus norvegicus dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Sus scrofa dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Sus scrofa dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Saccharomyces cerevisiae dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Saccharomyces cerevisiae dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Bos taurus dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Bos taurus dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Crithidia fasciculata dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Crithidia fasciculata dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Aedes aegypti dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Aedes aegypti dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Candida albicans dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Candida albicans dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Plasmodium falciparum dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Plasmodium falciparum dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Leishmania major dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Leishmania major dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Brugia pahangi dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Brugia pahangi dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Angiostrongylus cantonensis dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP
-
Toxoplasma gondii dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP
-
Toxoplasma gondii dihydrofolate + dTMP
-
r
5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Mus musculus dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Homo sapiens dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Angiostrongylus cantonensis dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Homo sapiens dihydrofolate + dTMP
-
?
5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Angiostrongylus cantonensis dihydrofolate + dTMP
-
?
additional information
-
Homo sapiens ?
-
?
additional information bifunctional enzyme dihydrofolate reductase-thymidylate synthase Plasmodium falciparum ?
-
?
additional information bifunctional enzyme dihydrofolate reductase-thymidylate synthase Leishmania major ?
-
?
additional information bifunctional enzyme dihydrofolate reductase-thymidylate synthase Toxoplasma gondii ?
-
?

Subunits

Subunits Comment Organism
dimer
-
Mus musculus
dimer
-
Homo sapiens
dimer
-
Rattus norvegicus
dimer
-
Saccharomyces cerevisiae
dimer
-
Bos taurus
dimer
-
Plasmodium falciparum
dimer
-
Leishmania major
dimer 2 * 31000, SDS-PAGE Angiostrongylus cantonensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000021
-
5-fluoro-2'-deoxyuridylic acid 50 nM Angiostrongylus cantonensis
0.0000235
-
5-fluoro-2'-deoxyuridylic acid
-
Angiostrongylus cantonensis
0.000026
-
5-fluoro-2'-deoxyuridylic acid 200 nM Angiostrongylus cantonensis
0.000833
-
trimethoprim 1.0 mM Angiostrongylus cantonensis
0.00087
-
trimethoprim 2.0 mM Angiostrongylus cantonensis
0.001
-
thymidine phosphate 0.25 mM, noncompetitive inhibition, 5,10-mehylenetetrahydrofolate as variable substrate Angiostrongylus cantonensis
0.00124
-
thymidine phosphate 1.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate Angiostrongylus cantonensis
0.0014
-
dihydrofolate 1.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate Angiostrongylus cantonensis
0.00178
-
dihydrofolate 5.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate Angiostrongylus cantonensis
0.00294
-
dihydrofolate 0.5 mM, noncompetitive inhibition, dUMP as variable substrate Angiostrongylus cantonensis
0.00303
-
dihydrofolate 1.0 mM, noncompetitive inhibition, dUMP as variable substrate Angiostrongylus cantonensis
0.238
-
methotrexate 0.05 mM Angiostrongylus cantonensis
0.267
-
methotrexate 0.02 mM Angiostrongylus cantonensis
0.3125
-
thymidine phosphate 0.25 mM, competitive inhibition, dUMP as variable substrate Angiostrongylus cantonensis
0.333
-
thymidine phosphate
-
Angiostrongylus cantonensis
0.406
-
methotrexate 0.5 mM Angiostrongylus cantonensis
0.413
-
methotrexate 1.0 mM Angiostrongylus cantonensis
0.852
-
trimethoprim
-
Angiostrongylus cantonensis
5
-
aminofolate 1.0 mM Angiostrongylus cantonensis
5.2
-
aminofolate 5.0 mM Angiostrongylus cantonensis