Crystallization (Comment) | Organism |
---|---|
structure of EgtD as an apoprotein (1.75 A resolution), as a binary complex with N-alpha-dimethylhistidine (1.9 A), and as a ternary complex with N-alpha-dimethylhistidine and SAH (1.5 A) | Mycolicibacterium smegmatis |
Protein Variants | Comment | Organism |
---|---|---|
E285A | active site mutation, change the substrate specificity and transforms the histidine-methyltransferase towards a tryptophan-methyltransferase | Mycolicibacterium smegmatis |
M252A/E285A | active site mutation, change the substrate specificity and transforms the histidine-methyltransferase towards a tryptophan-methyltransferase | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine + L-histidine | Mycolicibacterium smegmatis | - |
3 S-adenosyl-L-homocysteine + hercynine | - |
? | |
3 S-adenosyl-L-methionine + L-histidine | Mycolicibacterium smegmatis ATCC 700084 | - |
3 S-adenosyl-L-homocysteine + hercynine | - |
? | |
S-adenosyl-L-methionine + L-histidine | Mycolicibacterium smegmatis | - |
S-adenosyl-L-homocysteine + Nalpha-methyl-L-histidine | - |
? | |
S-adenosyl-L-methionine + L-histidine | Mycolicibacterium smegmatis ATCC 700084 | - |
S-adenosyl-L-homocysteine + Nalpha-methyl-L-histidine | - |
? | |
S-adenosyl-L-methionine + Nalpha,Nalpha-dimethyl-L-histidine | Mycolicibacterium smegmatis | - |
S-adenosyl-L-homocysteine + hercynine | - |
? | |
S-adenosyl-L-methionine + Nalpha,Nalpha-dimethyl-L-histidine | Mycolicibacterium smegmatis ATCC 700084 | - |
S-adenosyl-L-homocysteine + hercynine | - |
? | |
S-adenosyl-L-methionine + Nalpha-methyl-L-histidine | Mycolicibacterium smegmatis | - |
S-adenosyl-L-homocysteine + Nalpha,Nalpha-dimethyl-L-histidine | - |
? | |
S-adenosyl-L-methionine + Nalpha-methyl-L-histidine | Mycolicibacterium smegmatis ATCC 700084 | - |
S-adenosyl-L-homocysteine + Nalpha,Nalpha-dimethyl-L-histidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0R5M8 | - |
- |
Mycolicibacterium smegmatis ATCC 700084 | A0R5M8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine + L-histidine | - |
Mycolicibacterium smegmatis | 3 S-adenosyl-L-homocysteine + hercynine | - |
? | |
3 S-adenosyl-L-methionine + L-histidine | - |
Mycolicibacterium smegmatis ATCC 700084 | 3 S-adenosyl-L-homocysteine + hercynine | - |
? | |
additional information | EgtD is a four- and 70-times stronger binder of Nalpha-methylhistidine and N-alpha-dimethylhistidine, respectively, than of histidine | Mycolicibacterium smegmatis | ? | - |
- |
|
additional information | EgtD is a four- and 70-times stronger binder of Nalpha-methylhistidine and N-alpha-dimethylhistidine, respectively, than of histidine | Mycolicibacterium smegmatis ATCC 700084 | ? | - |
- |
|
S-adenosyl-L-methionine + DOPA | poor substrate | Mycolicibacterium smegmatis | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + L-histidine | - |
Mycolicibacterium smegmatis | S-adenosyl-L-homocysteine + Nalpha-methyl-L-histidine | - |
? | |
S-adenosyl-L-methionine + L-histidine | - |
Mycolicibacterium smegmatis ATCC 700084 | S-adenosyl-L-homocysteine + Nalpha-methyl-L-histidine | - |
? | |
S-adenosyl-L-methionine + L-phenylalanine | poor substrate | Mycolicibacterium smegmatis | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + L-tryptophan | poor substrate | Mycolicibacterium smegmatis | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + L-tyrosine | poor substrate | Mycolicibacterium smegmatis | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + Nalpha,Nalpha-dimethyl-L-histidine | - |
Mycolicibacterium smegmatis | S-adenosyl-L-homocysteine + hercynine | - |
? | |
S-adenosyl-L-methionine + Nalpha,Nalpha-dimethyl-L-histidine | - |
Mycolicibacterium smegmatis ATCC 700084 | S-adenosyl-L-homocysteine + hercynine | - |
? | |
S-adenosyl-L-methionine + Nalpha-methyl-L-histidine | - |
Mycolicibacterium smegmatis | S-adenosyl-L-homocysteine + Nalpha,Nalpha-dimethyl-L-histidine | - |
? | |
S-adenosyl-L-methionine + Nalpha-methyl-L-histidine | - |
Mycolicibacterium smegmatis ATCC 700084 | S-adenosyl-L-homocysteine + Nalpha,Nalpha-dimethyl-L-histidine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EgtD | - |
Mycolicibacterium smegmatis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
L-histidine | mutant E285A, pH 8.0, 26°C | Mycolicibacterium smegmatis | |
0.23 | - |
Nalpha-methyl-L-histidine | wild-type, pH 8.0, 26°C | Mycolicibacterium smegmatis | |
0.43 | - |
Nalpha,Nalpha-dimethyl-L-histidine | wild-type, pH 8.0, 26°C | Mycolicibacterium smegmatis | |
0.58 | - |
L-histidine | wild-type, pH 8.0, 26°C | Mycolicibacterium smegmatis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
L-tyrosine | wild-type, pH 8.0, 26°C | Mycolicibacterium smegmatis | |
0.0019 | - |
L-histidine | mutant M252A/E285A, pH 8.0, 26°C | Mycolicibacterium smegmatis | |
0.002 | - |
L-tryptophan | wild-type, pH 8.0, 26°C | Mycolicibacterium smegmatis | |
0.0024 | - |
L-phenylalanine | wild-type, pH 8.0, 26°C | Mycolicibacterium smegmatis | |
0.017 | - |
L-tyrosine | mutant M252A/E285A, pH 8.0, 26°C | Mycolicibacterium smegmatis | |
0.04 | - |
L-histidine | mutant E285A, pH 8.0, 26°C | Mycolicibacterium smegmatis | |
0.1 | - |
L-phenylalanine | mutant M252A/E285A, pH 8.0, 26°C | Mycolicibacterium smegmatis | |
0.4 | - |
L-tryptophan | mutant E285A, pH 8.0, 26°C | Mycolicibacterium smegmatis | |
5.3 | - |
L-histidine | wild-type, pH 8.0, 26°C | Mycolicibacterium smegmatis | |
5.5 | - |
L-tryptophan | mutant M252A/E285A, pH 8.0, 26°C | Mycolicibacterium smegmatis | |
13 | - |
Nalpha-methyl-L-histidine | wild-type, pH 8.0, 26°C | Mycolicibacterium smegmatis | |
17 | - |
Nalpha,Nalpha-dimethyl-L-histidine | wild-type, pH 8.0, 26°C | Mycolicibacterium smegmatis |