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Literature summary for 2.1.1.41 extracted from

  • Haubrich, B.; Collins, E.; Howard, A.; Wang, Q.; Snell, W.; Miller, M.; Thomas, C.; Pleasant, S.; Nes, W.
    Characterization, mutagenesis and mechanistic analysis of an ancient algal sterol C24-methyltransferase Implications for understanding sterol evolution in the green lineage (2015), Phytochemistry, 113, 64-72 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.41

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 24(28)-methylene cycloartenol pH 7.5, 30°C Chlamydomonas reinhardtii
0.02
-
 obtusifoliol pH 7.5, 30°C Chlamydomonas reinhardtii
0.022
-
 24(28)-methylene lophenol pH 7.5, 30°C Chlamydomonas reinhardtii
0.024
-
 lanosterol pH 7.5, 30°C Chlamydomonas reinhardtii
0.025
-
 Cycloartenol pH 7.5, 30°C Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii A8IJ34
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme catalyzes both the reactions of EC 2.1.1.142, cycloartenol 24-C-methyltransferase and EC 2.1.1.143, 24-methylenesterol C-methyltransferase. SMT1 catalyzes a sterol methylation pathway by the algal Delta25(27)-olefin route, where methylation proceeds by a conserved SN2 reaction and deprotonation proceeds from the pro-Z methyl group on lanosterol corresponding to C27 Chlamydomonas reinhardtii ?
-
-
S-adenosyl-L-methionine + 24(28)-methylene cycloartenol
-
 Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + 24(28)-ethylidene cycloartenol
-
 ?
S-adenosyl-L-methionine + 24(28)-methylene lophenol
-
 Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + 24(28)-ethylidene lophenol
-
 ?
S-adenosyl-L-methionine + cycloartenol reaction of EC 2.1.1.142 Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + cyclolaudenol + 24(28)-methylene cycloartanol
-
 ?
S-adenosyl-L-methionine + lanosterol reaction of EC 2.1.1.142 Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + 24beta-methyl lanosta-8,25(27)-enol + 24(28)-methylene lanosterol
-
 ?
S-adenosyl-L-methionine + obtusifoliol
-
 Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + ?
-
 ?
S-adenosyl-L-methionine + zymosterol
-
 Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + fecosterol
-
 ?

Synonyms

Synonyms Comment Organism
SMT1
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
 Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Chlamydomonas reinhardtii