Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
24(28)-methylene cycloartenol | pH 7.5, 30°C | Chlamydomonas reinhardtii | |
0.02 | - |
obtusifoliol | pH 7.5, 30°C | Chlamydomonas reinhardtii | |
0.022 | - |
24(28)-methylene lophenol | pH 7.5, 30°C | Chlamydomonas reinhardtii | |
0.024 | - |
lanosterol | pH 7.5, 30°C | Chlamydomonas reinhardtii | |
0.025 | - |
Cycloartenol | pH 7.5, 30°C | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | A8IJ34 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme catalyzes both the reactions of EC 2.1.1.142, cycloartenol 24-C-methyltransferase and EC 2.1.1.143, 24-methylenesterol C-methyltransferase. SMT1 catalyzes a sterol methylation pathway by the algal Delta25(27)-olefin route, where methylation proceeds by a conserved SN2 reaction and deprotonation proceeds from the pro-Z methyl group on lanosterol corresponding to C27 | Chlamydomonas reinhardtii | ? | - |
- |
|
S-adenosyl-L-methionine + 24(28)-methylene cycloartenol | - |
Chlamydomonas reinhardtii | S-adenosyl-L-homocysteine + 24(28)-ethylidene cycloartenol | - |
? | |
S-adenosyl-L-methionine + 24(28)-methylene lophenol | - |
Chlamydomonas reinhardtii | S-adenosyl-L-homocysteine + 24(28)-ethylidene lophenol | - |
? | |
S-adenosyl-L-methionine + cycloartenol | reaction of EC 2.1.1.142 | Chlamydomonas reinhardtii | S-adenosyl-L-homocysteine + cyclolaudenol + 24(28)-methylene cycloartanol | - |
? | |
S-adenosyl-L-methionine + lanosterol | reaction of EC 2.1.1.142 | Chlamydomonas reinhardtii | S-adenosyl-L-homocysteine + 24beta-methyl lanosta-8,25(27)-enol + 24(28)-methylene lanosterol | - |
? | |
S-adenosyl-L-methionine + obtusifoliol | - |
Chlamydomonas reinhardtii | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + zymosterol | - |
Chlamydomonas reinhardtii | S-adenosyl-L-homocysteine + fecosterol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SMT1 | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Chlamydomonas reinhardtii |