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Literature summary for 2.1.1.41 extracted from

  • Haubrich, B.A.; Collins, E.K.; Howard, A.L.; Wang, Q.; Snell, W.J.; Miller, M.B.; Thomas, C.D.; Pleasant, S.K.; Nes, W.D.
    Characterization, mutagenesis and mechanistic analysis of an ancient algal sterol C24-methyltransferase: Implications for understanding sterol evolution in the green lineage (2015), Phytochemistry, 113, 64-72.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.41

Protein Variants

Protein Variants Comment Organism
Y110F no significant change in products Chlamydomonas reinhardtii
Y110L remodeling the active site to alter the electronics results in delayed timing of the hydride migration from methyl attack of the DELTA24-bond, that thereby produces metabolic switching of product ratios in favor of DELTA25(27)-olefins or impairs the second C1-transfer activity Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii A8IJ34
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
24(28)-methylenecycloartanol + S-adenosyl-L-methionine
-
 Chlamydomonas reinhardtii ? + S-adenosyl-L-homocysteine ratio of DELTA25(27) to DELTA24(28)-product for wild-type is 100:0, 35% conversion ?
24(28)-methylenelophenol + S-adenosyl-L-methionine
-
 Chlamydomonas reinhardtii ?
-
 ?
cycloartenol + S-adenosyl-L-methionine
-
 Chlamydomonas reinhardtii 24(28)-methylenecycloartanol + S-adenosyl-L-homocysteine ratio of DELTA25(27) to DELTA24(28)-product for wild-type is 57:43, 70% conversion ?
lanosterol + S-adenosyl-L-methionine
-
 Chlamydomonas reinhardtii 24beta-methyllanosta-8,25(27)-enol + 24(28)-methylenelanosterol + S-adenosyl-L-homocysteine products are 55% 24beta-methyllanosta-8,25(27)-enol, 38% 24(28)-methylenelanosterol (eburicol), and 7% 24beta-ethyllanosta-8,25(27)-enol ?
additional information enzyme catalyzes a sterol methylation pathway by the algal DELTA25(27)-olefin route, where methylation proceeds by a conserved SN2 reaction and de-protonation proceeds from the pro-Z methyl group on lanosterol corresponding to C27 Chlamydomonas reinhardtii ?
-
 ?
obtusifoliol + S-adenosyl-L-methionine
-
 Chlamydomonas reinhardtii ? + S-adenosyl-L-homocysteine
-
 ?
zymosterol + S-adenosyl-L-methionine
-
 Chlamydomonas reinhardtii fecosterol + S-adenosyl-L-homocysteine ratio of DELTA25(27) to DELTA24(28)-product for wild-type is 28:72, 70% conversion ?

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.018
-
 24(28)-methylenecycloartanol pH not specified in the publication, temperature not specified in the publication Chlamydomonas reinhardtii
0.019
-
 obtusifoliol pH not specified in the publication, temperature not specified in the publication Chlamydomonas reinhardtii
0.02
-
 24(28)-methylenelophenol pH not specified in the publication, temperature not specified in the publication Chlamydomonas reinhardtii
0.022
-
 Zymosterol pH not specified in the publication, temperature not specified in the publication Chlamydomonas reinhardtii
0.024
-
 lanosterol pH not specified in the publication, temperature not specified in the publication Chlamydomonas reinhardtii
0.025
-
 Cycloartenol pH not specified in the publication, temperature not specified in the publication Chlamydomonas reinhardtii