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Literature summary for 2.1.1.41 extracted from

  • Lin, H.K.; Knoche, H.W.
    A sterol methyltransferase from bean rust uredospores Uromyces phaseoli (1976), Phytochemistry, 15, 683-687.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
NAD+
-
Uromyces phaseoli
NADP+
-
Uromyces phaseoli
Triparanol
-
Uromyces phaseoli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0334
-
S-adenosyl-L-methionine
-
Uromyces phaseoli
0.055 0.075 Zymosterol value depends on concentration of detergent Uromyces phaseoli

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane some activity Uromyces phaseoli 5743
-
mitochondrion major activity Uromyces phaseoli 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
zymosterol + S-adenosyl-L-methionine Uromyces phaseoli
-
fecosterol + S-adenosyl-L-homocysteine
-
?

Organism

Organism UniProt Comment Textmining
Uromyces phaseoli
-
bean rust
-

Purification (Commentary)

Purification (Comment) Organism
partial Uromyces phaseoli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Uromyces phaseoli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
24-methylenecholesterol + S-adenosyl-L-methionine 5% the reaction rate of zymosterol Uromyces phaseoli 24-ethylidenecholesterol + S-adenosyl-L-homocysteine
-
?
desmosterol + S-adenosyl-L-methionine desmosterol is 5alpha-cholesta-5,24-dien-3beta-ol Uromyces phaseoli 24-methylenedesmosterol + S-adenosyl-L-homocysteine
-
?
lanosterol + S-adenosyl-L-methionine lanosterol is 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3-ol Uromyces phaseoli 24-methylene-24,25-dihydrolanosterol + S-adenosyl-L-homocysteine
-
?
zymosterol + S-adenosyl-L-methionine
-
Uromyces phaseoli fecosterol + S-adenosyl-L-homocysteine
-
?
zymosterol + S-adenosyl-L-methionine zymosterol is 5alpha-cholesta-8,24-dien-3beta-ol Uromyces phaseoli fecosterol + S-adenosyl-L-homocysteine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
-
Uromyces phaseoli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 30 less than half-maximal activity above and below Uromyces phaseoli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.7
-
Uromyces phaseoli

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5
-
Uromyces phaseoli