Literature summary for 2.1.1.37 extracted from

Meister, G.E.; Chandrasegaran, S.; Ostermeier, M.
Heterodimeric DNA methyltransferases as a platform for creating designer zinc finger methyltransferases for targeted DNA methylation in cells (2009), Nucleic Acids Res., 38, 1749-1759.

Search for more literature for 2.1.1.37

Cloned(Commentary)

Cloned (Comment)	Organism
construction of pDIM-N7 and pAR for expression of M.EcoHK31I fragments, construction of pDIM-N7 Tyr123-alpha and pAR beta-Tyr456 plasmids for combinatorial library, the recombinantly expressed M.EcoHK31I enzyme is functional in vivo when the two fragments are encoded as separate genes, overview	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
additional information	removal of 50 amino acids from the N-terminus of the beta fragment of M.EcoHK31I severely reduces but does not eliminate methylation activity in vivo. Design of the methylation target site and fusion of the a and betaDELTA42 fragments to zinc fingers	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	methylation specificity is affected by gene copy number and induction levels	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
DNA + S-adenosyl-L-methionine	Escherichia coli	-	DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + S-adenosyl-L-methionine	-	Escherichia coli	DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine	-	?
DNA + S-adenosyl-L-methionine	DNA methyltransferase M.EcoHK31I methylates the inner cytosine of 5'-YGGCCR-3'	Escherichia coli	DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine	-	?
additional information	site-based methylation is dependent on the relative position of the zinc finger binding sites and the target methylation site, methylation specificity for the target site also depends on zinc finger binding	Escherichia coli	?	-	?

Subunits

Subunits	Comment	Organism
heterodimer	DNA methyltransferase M.EcoHK31I is a natural heterodimer	Escherichia coli

Synonyms

Synonyms	Comment	Organism
DNA methyltransferase	-	Escherichia coli
M.EcoHK31l	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Escherichia coli

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Release 2023.1 (January 2023)