Cloned (Comment) | Organism |
---|---|
expression of His6-tagged SssI in Escherichia coli strain ER1821 | Synechocystis sp. |
Protein Variants | Comment | Organism |
---|---|---|
C141S | site-directed mutagenesis in motif IV | Synechocystis sp. |
C368A | the mutation does not affect the enzyme activity | Synechocystis sp. |
E186A | site-directed mutagenesis in motif VI, almost inactive mutant | Synechocystis sp. |
K297A | site-directed mutagenesis in motif TRD, the mutant is resistant to Hin6I digestion | Synechocystis sp. |
N299A | site-directed mutagenesis in motif TRD, the mutant is resistant to Hin6I digestion | Synechocystis sp. |
Q147L | site-directed mutagenesis in motif IV, the mutant shows less than 10% of wild-type activity | Synechocystis sp. |
R230A | site-directed mutagenesis in motif VIII, almost inactive mutant | Synechocystis sp. |
R232A | site-directed mutagenesis in motif VIII, the mutant shows less than 10% of wild-type activity | Synechocystis sp. |
S145A | site-directed mutagenesis in motif IV, the mutant shows less than 10% of wild-type activity | Synechocystis sp. |
S2C/C141S/C368A | replacement of the active site Cys141 reduces the enzyme activity, while the substitutions Ser2Cys and Cys368Ala as well as a C-terminal Ser-His6 tag do not affect the enzyme activity | Synechocystis sp. |
S317A | site-directed mutagenesis in motif TRD, the mutant shows about 50% of wild-type activity | Synechocystis sp. |
T313A | site-directed mutagenesis in motif TRD, the mutant is resistant to Hin6I digestion | Synechocystis sp. |
T313D | site-directed mutagenesis in motif TRD, almost inactive mutant | Synechocystis sp. |
T313H | site-directed mutagenesis in motif TRD, almost inactive mutant | Synechocystis sp. |
V188X | replacement of the conserved Val188 from the ENV motif VI results in a 5fold increased DNA binding affinity and a 2fold decreased catalytic activity | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone | - |
Synechocystis sp. | |
oligodeoxyribonucleotides containing 2-aminopurine | - |
Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | recombinant kinetics of wild-type and mutant enzymes, overview | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | Synechocystis sp. | M.SssI is the only known prokaryotic C5-MTase, which recognizes the short sequence CG and thus has the same specificity as mammalian MTases | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged SssI from Escherichia coli strain ER1821 by cobalt affinity chromatography | Synechocystis sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + DNA containing cytosine = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | catalytic reaction mechanism | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | M.SssI is the only known prokaryotic C5-MTase, which recognizes the short sequence CG and thus has the same specificity as mammalian MTases | Synechocystis sp. | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? | |
DNA + S-adenosyl-L-methionine | structural basis for DNA recognition and base flipping, overview | Synechocystis sp. | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C5-MTase | - |
Synechocystis sp. |
CG recognizing DNA methyltransferase SssI | - |
Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Synechocystis sp. |