Literature summary for 2.1.1.37 extracted from

  • van Bemmel, D.M.; Brank, A.S.; Eritja, R.; Marquez, V.E.; Christman, J.K.
    DNA (Cytosine-C5) methyltransferase inhibition by oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone (zebularine aglycon) at the enzymatic target site (2009), Biochem. Pharmacol., 78, 633-641.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
M.HhaI overexpression in Escherichia coli strain ER1727 Haemophilus parahaemolyticus

Inhibitors

Inhibitors Comment Organism Structure
2-(1H)-pyrimidinone riboside i.e. zebularine, is a more stable, less cytotoxic inhibitor compared to 5-azacytidine probably due to differing stability and reversibility of the covalent bonds. The ternary complexes between the enzyme, 2-(1H)-pyrimidinone inhibitor, and the cofactor S-adenosyl-L-methionine are maintained through the formation of a reversible covalent interaction Haemophilus parahaemolyticus
5-azacytidine zebularine is a more stable, less cytotoxic inhibitor compared to 5-azacytidine probably due to differing stability and reversibility of the covalent bonds Haemophilus parahaemolyticus
additional information inhibitor binding kinetics Haemophilus parahaemolyticus
oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone at the enzymatic target site are competitive inhibitors of both prokaryotic and mammalian DNA C5 methyltransferases, overview Haemophilus parahaemolyticus
oligodeoxyribonucleotides containing 5-azacytidine 5-azacytosine oligodeoxyribonucleotides form complexes with C5 DNA methyltransferases that are irreversible when the 5-azacytosine ring is intact, overview Haemophilus parahaemolyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + S-adenosyl-L-methionine Haemophilus parahaemolyticus
-
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
?

Organism

Organism UniProt Comment Textmining
Haemophilus parahaemolyticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + S-adenosyl-L-methionine
-
Haemophilus parahaemolyticus DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
?

Synonyms

Synonyms Comment Organism
M.HhaI
-
Haemophilus parahaemolyticus
DNA (cytosine-C5) methyltransferase
-
Haemophilus parahaemolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Haemophilus parahaemolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Haemophilus parahaemolyticus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine the ternary complexes between the enzyme, 2-(1H)-pyrimidinone inhibitor, and the cofactor S-adenosyl-L-methionine are maintained through the formation of a reversible covalent interaction Haemophilus parahaemolyticus