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Literature summary for 2.1.1.37 extracted from
- Murine DNA (cytosine-5-)methyltransferase: steady-state and substrate trapping analyses of the kinetic mechanism (1989), Biochemistry, 37, 15162-15169.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000125 | - |
poly(dI*dC-dI*dC) of chain length 2000 | - |
Mus musculus | |
| 0.00014 | - |
poly(dI*dC-dI*dC) of chain length 5000 | - |
Mus musculus | |
| 0.0003 | - |
poly(dI*dC-dI*dC) of chain length 500 | - |
Mus musculus | |
| 0.00189 | - |
poly(dI*dC-dI*dC) of chain length 100 | - |
Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mus musculus | the enzyme is essential for viable mammalian development and has a central function in the determination and maintenance of epigenetic methalation pattern | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-methionine + DNA containing cytosine = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | sequential ordered bi-bi kinetic mechanism in which DNA binds to the enzyme first, followed by S-adenosyl-L-homocysteine and methylated DNA are released, formation of an enzyme-DNA-DNA ternary complex | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythroleukemia cell | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is essential for viable mammalian development and has a central function in the determination and maintenance of epigenetic methalation pattern | Mus musculus | ? | - |
? | |
| S-adenosyl-L-methionine + DNA | - |
Mus musculus | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? | |
| S-adenosyl-L-methionine + DNA | poly(dI*dC-dI*dC) of chain length 100, 500, 2000 or 5000 base pairs | Mus musculus | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? | |
| S-adenosyl-L-methionine + poly(dI*dC-dI*dC) of chain length 100 | - |
Mus musculus | ? | - |
? | |
| S-adenosyl-L-methionine + poly(dI*dC-dI*dC) of chain length 2000 | - |
Mus musculus | ? | - |
? | |
| S-adenosyl-L-methionine + poly(dI*dC-dI*dC) of chain length 500 | - |
Mus musculus | ? | - |
? | |
| S-adenosyl-L-methionine + poly(dI*dC-dI*dC) of chain length 5000 | - |
Mus musculus | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00597 | - |
poly(dI*dC-dI*dC) of chain length 100 | - |
Mus musculus | |
| 0.00633 | - |
poly(dI*dC-dI*dC) of chain length 500 | - |
Mus musculus | |
| 0.00692 | - |
poly(dI*dC-dI*dC) of chain length 2000 | - |
Mus musculus | |
| 0.00867 | - |
poly(dI*dC-dI*dC) of chain length 5000 | - |
Mus musculus |
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