Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATF7IP | SETDB1 partner protein. SETDB1 and the SETDB1:ATF7IP complex efficiently catalyze both monomethylation and dimethylation of H3K9 peptide substrates. The activity of the binary complex is 4fold lower than SETDB1 alone | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
[histone H3 peptide 1-15]-L-lysine9 | SETDB1:ATF7IP complex, pH 7.6, 23°C | Homo sapiens | |
0.41 | - |
[histone H3 peptide 1-15]-L-lysine9 | pH 7.6, 23°C | Homo sapiens | |
0.41 | - |
[histone H3 peptide 1-15]-N6-methyl-L-lysine9 | SETDB1:ATF7IP complex, pH 7.6, 23°C | Homo sapiens | |
0.53 | - |
S-adenosyl-L-methionine | substrate [histoneH3 peptide 1-15]-L-lysine9, SETDB1:ATF7IP complex, pH 7.6, 23°C | Homo sapiens | |
0.54 | - |
S-adenosyl-L-methionine | substrate [histoneH3 peptide 1-15]-N6-methyl-L-lysine9, SETDB1:ATF7IP complex, pH 7.6, 23°C | Homo sapiens | |
0.56 | - |
S-adenosyl-L-methionine | substrate [histoneH3 peptide 1-15]-N6-methyl-L-lysine9, pH 7.6, 23°C | Homo sapiens | |
0.59 | - |
[histone H3 peptide 1-15]-N6-methyl-L-lysine9 | pH 7.6, 23°C | Homo sapiens | |
0.68 | - |
S-adenosyl-L-methionine | substrate [histoneH3 peptide 1-15]-L-lysine9, pH 7.6, 23°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q15047 | cf. EC 2.1.1.366 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | H3K9 methylation by SETDB1 occurs in a distributive manner | Homo sapiens | ? | - |
- |
|
S-adenosyl-L-methionine + [histone H3 peptide 1-15]-L-lysine9 | - |
Homo sapiens | S-adenosyl-L-homocysteine + [histone H3 peptide 1-15]-N6-methyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + [histone H3 peptide 1-15]-N6,N6-dimethyl-L-lysine9 | - |
Homo sapiens | S-adenosyl-L-homocysteine + [histone H3 peptide 1-15]-N6,N6,N6-trimethyl-L-lysine9 | - |
? | |
S-adenosyl-L-methionine + [histone H3 peptide 1-15]-N6-methyl-L-lysine9 | - |
Homo sapiens | S-adenosyl-L-homocysteine + [histone H3 peptide 1-15]-N6,N6-dimethyl-L-lysine9 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
[histone H3 peptide 1-15]-L-lysine9 | SETDB1:ATF7IP complex, pH 7.6, 23°C | Homo sapiens | |
0.045 | - |
[histone H3 peptide 1-15]-N6-methyl-L-lysine9 | SETDB1:ATF7IP complex, pH 7.6, 23°C | Homo sapiens | |
0.072 | - |
S-adenosyl-L-methionine | substrate [histone H3 peptide 1-15]-L-lysine9, SETDB1:ATF7IP complex, pH 7.6, 23°C | Homo sapiens | |
0.09 | - |
S-adenosyl-L-methionine | substrate [histone H3 peptide 1-15]-N6-methyl-L-lysine9, SETDB1:ATF7IP complex, pH 7.6, 23°C | Homo sapiens | |
0.192 | - |
[histone H3 peptide 1-15]-L-lysine9 | pH 7.6, 23°C | Homo sapiens | |
0.293 | - |
[histone H3 peptide 1-15]-N6-methyl-L-lysine9 | pH 7.6, 23°C | Homo sapiens | |
0.337 | - |
S-adenosyl-L-methionine | substrate [histone H3 peptide 1-15]-L-lysine9, pH 7.6, 23°C | Homo sapiens | |
0.352 | - |
S-adenosyl-L-methionine | substrate [histone H3 peptide 1-15]-N6-methyl-L-lysine9, pH 7.6, 23°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
[histone H3 peptide 1-15]-L-lysine9 | SETDB1:ATF7IP complex, pH 7.6, 23°C | Homo sapiens | |
0.11 | - |
[histone H3 peptide 1-15]-N6-methyl-L-lysine9 | SETDB1:ATF7IP complex, pH 7.6, 23°C | Homo sapiens | |
0.135 | - |
S-adenosyl-L-methionine | substrate [histoneH3 peptide 1-15]-L-lysine9, SETDB1:ATF7IP complex, pH 7.6, 23°C | Homo sapiens | |
0.167 | - |
S-adenosyl-L-methionine | substrate [histoneH3 peptide 1-15]-N6-methyl-L-lysine9, SETDB1:ATF7IP complex, pH 7.6, 23°C | Homo sapiens | |
0.467 | - |
[histone H3 peptide 1-15]-L-lysine9 | pH 7.6, 23°C | Homo sapiens | |
0.495 | - |
S-adenosyl-L-methionine | substrate [histoneH3 peptide 1-15]-L-lysine9, pH 7.6, 23°C | Homo sapiens | |
0.497 | - |
[histone H3 peptide 1-15]-N6-methyl-L-lysine9 | pH 7.6, 23°C | Homo sapiens | |
0.628 | - |
S-adenosyl-L-methionine | substrate [histoneH3 peptide 1-15]-N6-methyl-L-lysine9, pH 7.6, 23°C | Homo sapiens |