Literature summary for 2.1.1.361 extracted from

Nishioka, K.; Rice, J.C.; Sarma, K.; Erdjument-Bromage, H.; Werner, J.; Wang, Y.; Chuikov, S.; Valenzuela, P.; Tempst, P.; Steward, R.; Lis, J.T.; Allis, C.D.; Reinberg, D.
PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin (2002), Mol. Cell, 9, 1201-1213 .

Search for more literature for 2.1.1.361

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)pLysS cells	Drosophila melanogaster
expressed in Escherichia coli BL21(DE3)pLysS cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
R265G	inactive	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	5 mM used in assay conditions	Drosophila melanogaster
Ca2+	5 mM used in assay conditions	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	gel filtration	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + a [histone H4]-L-lysine20	Drosophila melanogaster	-	S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20	-	?
S-adenosyl-L-methionine + a [histone H4]-L-lysine20	Homo sapiens	-	S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20	-	?

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-
Homo sapiens	Q9NQR1	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-cellulose column chromatography, Superose 6 column chromatography, Mono S column chromatography, and heparin agarose column chromatography	Drosophila melanogaster
DEAE-cellulose column chromatography, Superose 6 column chromatography, Mono S column chromatography, and heparin agarose column chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
HeLa cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + a [histone H4]-L-lysine20	-	Drosophila melanogaster	S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20	-	?
S-adenosyl-L-methionine + a [histone H4]-L-lysine20	-	Homo sapiens	S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 40000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
H4-K20-specific HMT	-	Drosophila melanogaster
H4-K20-specific HMT	-	Homo sapiens
histone H4 lysine 20 methyltransferase	-	Drosophila melanogaster
histone H4 lysine 20 methyltransferase	-	Homo sapiens
Pr-Set7	-	Drosophila melanogaster
Pr-Set7	-	Homo sapiens

General Information

General Information	Comment	Organism
metabolism	enzyme-mediated methylation of histone H4 lysine 20 maintains silent chromatin, in part, by precluding neighboring acetylation on the histone H4 tail	Homo sapiens
physiological function	methylation of histone H4 lysine 20 by the enzyme is essential for development and viability in Drosophila	Drosophila melanogaster

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Release 2023.1 (January 2023)