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Literature summary for 2.1.1.360 extracted from

  • Zhang, W.; Xia, X.; Jalal, D.I.; Kuncewicz, T.; Xu, W.; Lesage, G.D.; Kone, B.C.
    Aldosterone-sensitive repression of ENaCalpha transcription by a histone H3 lysine-79 methyltransferase (2006), Am. J. Physiol. Cell Physiol., 290, C936-C946.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.360

Application

Application Comment Organism
additional information mDot1a is a novel aldosterone regulated histone modification enzyme, and, through binding the ENaCalpha promoter and hypermethylating histone H3 K79 associated with the ENaCalpha promoter, a negative regulator of ENaCalpha transcription Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
into vector pcDNA3.1 (+) Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information methyltransferase-dead mutant pEGFP-mDot1aRCR, mENaCalpha mRNA levels in cells transfected with the methyltransferase-dead mutant are almost twice that of vector-transfected cells, knockdown of mDot1a mRNA levels by RNA interference augments endogenous mENaCalpha expression and activity of mENaCalpha-promoter luciferase reporter Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
aldosterone
-
 Homo sapiens
aldosterone at an early time point (2 h) downregulates the steady-state level of mDot1a mRNA and this precedes, and is associated with, downregulation of histone H3 K79 methylation in mIMCD3 cells Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
 Western blot analysis, mDot1a in the kidney Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
epithelial cell
-
 Mus musculus
-
HEK-293T cell
-
 Homo sapiens
-
kidney
-
 Mus musculus
-
additional information mIMCD3 cell, mDot1a expressed and maintained at low levels Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information mDot1a directly or indirectly associates with and hypermethylated histone H3 K79 at specific portions of the ENaCalpha 5'-flanking region, inducing a local alteration of the chromatin structure that facilitates ENaCalpha mRNA expression Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
histone H3 lysine-79 methyltransferase
-
 Mus musculus
histone H3 lysine-79 methyltransferase
-
 Homo sapiens
mDot1a
-
 Mus musculus
mDot1a
-
 Homo sapiens
murine disruptor of telomeric silencing alternative splice variant a
-
 Mus musculus
murine disruptor of telomeric silencing alternative splice variant a
-
 Homo sapiens