Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with BIX-01294, hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 18-20% (w/v) polyethylene glycol 4000 and 7-10% (v/v) isopropanol, in the absence or presence of DMSO (6-36% v/v) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BIX-01294 | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel-chelating column chromatography, HiTrap-Q column chromatography, and Superdex-75 and -200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA methyltransferase 1 | - |
Homo sapiens | ? | - |
? | |
S-adenosyl-L-methionine + histone H3(K9) | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G9a | - |
Homo sapiens |
G9a-like protein lysine methyltransferase | - |
Homo sapiens |
GLP | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0007 | - |
G9a-like protein lysine methyltransferase, in 20 mM Tris (pH 8.5), at 30°C | Homo sapiens | BIX-01294 | |
0.0019 | - |
histone lysine methyltransferase G9a, in 20 mM Tris (pH 8.5), at 30°C | Homo sapiens | BIX-01294 |