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Literature summary for 2.1.1.35 extracted from

  • Carter, J.M.; Emmett, W.; Rdl Mozos, I.; Kotter, A.; Helm, M.; Ule, J.; Hussain, S.
    FICC-Seq A method for enzyme-specified profiling of methyl-5-uridine in cellular RNA (2019), Nucleic Acids Res., 47, e113 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.35

Application

Application Comment Organism
analysis genome-wide single-nucleotide resolution mapping method, Fluorouracil-Induced-Catalytic-Crosslinking-Sequencing (FICC-Seq), to identify the relevant enzymatic targets. FICC-Seq is a robust method for accurate and reliable detection of relevant enzymatic target sites Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5-fluorouracil exposure to 5-fluorouracil leads to extensive irreversible TRMT2A-tRNA crosslinking in vivo Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IZ69
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HAP-1 cell
-
 Homo sapiens
-
HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information TRMT2A commonly targets U54 of cytosolic tRNAs Homo sapiens ?
-
-
S-adenosyl-L-methionine + uracil54 in tRNA
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA
-
 ?

Synonyms

Synonyms Comment Organism
TRMT2A
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function TRMT2A is responsible for the majority of m5U present in human RNA, it commonly targets U54 of cytosolic tRNAs. In both HAP-1 and HEK-293 TRMT2A knockout cell lines, the majority of m5U signal present in total RNA from control cell lines is absent Homo sapiens