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Literature summary for 2.1.1.35 extracted from

  • Sengupta, R.; Vainauskas, S.; Yarian, C.; Sochacka, E.; Malkiewicz, A.; Guenther, R.H.; Koshlap, K.M.; Agris, P.F.
    Modified constructs of the tRNA TPsiC domain to probe substrate conformational requirements of m1A58 and m5U54 tRNA methyltransferases (2000), Nucleic Acids Res., 28, 1374-1380.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.35

Cloned(Commentary)

Cloned (Comment) Organism
trmA gene is cloned under control of the inducible lac promoter, DH5alpha Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 uracil54 in yeast tRNA-Phe
-
 Escherichia coli
0.0156
-
 uracil54 in unmodified yeast tRNA-Phe TPsiC-stem and loop heptadecamer
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + uridine54 in tRNA Escherichia coli tRNA modifying enzyme S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?
S-adenosyl-L-methionine + uridine54 in tRNA Escherichia coli GRB822 tRNA modifying enzyme S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli GRB822
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + uracil54 in unmodified yeast tRNA-Phe TPsiC-stem and loop heptadecamer
-
 Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil54 in unmodified yeast tRNA-Phe TPsiC-stem and loop heptadecamer
-
 ?
S-adenosyl-L-methionine + uracil54 in unmodified yeast tRNA-Phe TPsiC-stem and loop heptadecamer
-
 Escherichia coli GRB822 S-adenosyl-L-homocysteine + 5-methyluracil54 in unmodified yeast tRNA-Phe TPsiC-stem and loop heptadecamer
-
 ?
S-adenosyl-L-methionine + uracil54 in yeast tRNA-Phe
-
 Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA-Phe
-
 ?
S-adenosyl-L-methionine + uracil54 in yeast tRNA-Phe
-
 Escherichia coli GRB822 S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA-Phe
-
 ?
S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptors: methyl-deficient and T-deficient E. coli tRNA, enzyme recognizes and methylates TSL: a minimal unmodified yeast tRNAPhe TPsiC-stem and loop heptadecamer, composed of a 5 bp stem and seven-membered loop, recognition determinants are contained within the sequence, modification and secondary structure of TSL, enzyme is sensitive to conformational perturbations at or adjacent to the methylation site, positions 54 and 55 Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV ?
S-adenosyl-L-methionine + uridine54 in tRNA methylates U54 in the TPsiC-loop of tRNA Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV ?
S-adenosyl-L-methionine + uridine54 in tRNA tRNA modifying enzyme Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?
S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptors: methyl-deficient and T-deficient E. coli tRNA, enzyme recognizes and methylates TSL: a minimal unmodified yeast tRNAPhe TPsiC-stem and loop heptadecamer, composed of a 5 bp stem and seven-membered loop, recognition determinants are contained within the sequence, modification and secondary structure of TSL, enzyme is sensitive to conformational perturbations at or adjacent to the methylation site, positions 54 and 55 Escherichia coli GRB822 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV ?
S-adenosyl-L-methionine + uridine54 in tRNA methylates U54 in the TPsiC-loop of tRNA Escherichia coli GRB822 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA formation of 5-methyluridine in position 54 of tRNA, in the TPsiC sequence in loop IV ?
S-adenosyl-L-methionine + uridine54 in tRNA tRNA modifying enzyme Escherichia coli GRB822 S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Escherichia coli