Cloned (Comment) | Organism |
---|---|
trmA gene encodes enzyme, gene is essential for the organism | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-Fluorouracil substituted tRNA | potent inhibitor | Escherichia coli | |
tRNAPhe | inhibits binding of 16S rRNA to RUMT | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme also catalyzes exchange of tritium of [3H]-Ura-16S rRNA and protons of water | Escherichia coli | additional information | - |
? | |
S-adenosyl-L-methionine + 16S rRNA | methyl group acceptor: E. coli 16S rRNA, site of methylation is U788 which is within a 9-base loop of a predicted stem-loop structure, mechanism is proposed to involve formation of a covalent, reversible Michael adduct with the target U788, poor substrate: methylation of 16S rRNA 40fold slower than of tRNAPhe | Escherichia coli | S-adenosyl-L-homocysteine + 16S rRNA containing thymine | in vitro product m5U is not normally found in wild-type 16S rRNA, U788 is not highly methylated in vivo, 1 mol m5U per mol of 16S rRNA | ? | |
S-adenosyl-L-methionine + uridine54 in tRNA | substrate consensus sequence for the T-arm of tRNA consists of a 2-5 base-pair stem and a 7-base loop | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methylates U54 in the TPsiC-loop of tRNA | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |