Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Burkholderia glumae |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Burkholderia glumae |
Protein Variants | Comment | Organism |
---|---|---|
Y7A | structures of the mutant enzyme show an open active site, suggesting that the hydroxyl group of Tyr7 plays a role in opening and closing the active site during the multistep reaction | Burkholderia glumae |
Y7F | crystal structures of mutant enzyme consistently show a closed active site | Burkholderia glumae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 1,6-didemethyltoxoflavin | Burkholderia glumae | the enzyme is a dual-specificity methyltransferase that catalyses the last two steps of toxoflavin biosynthesis. Toxoflavin is a major virulence factor of several bacterial crop pathogens | S-adenosyl-L-homocysteine + reumycin | - |
? | |
S-adenosyl-L-methionine + reumycin | Burkholderia glumae | the enzyme is a dual-specificity methyltransferase that catalyses the last two steps of toxoflavin biosynthesis. Toxoflavin is a major virulence factor of several bacterial crop pathogens | S-adenosyl-L-homocysteine + toxoflavin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia glumae | Q9LBJ0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Burkholderia glumae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 1,6-didemethyltoxoflavin | - |
Burkholderia glumae | S-adenosyl-L-homocysteine + reumycin | - |
? | |
S-adenosyl-L-methionine + 1,6-didemethyltoxoflavin | the enzyme is a dual-specificity methyltransferase that catalyses the last two steps of toxoflavin biosynthesis. Toxoflavin is a major virulence factor of several bacterial crop pathogens | Burkholderia glumae | S-adenosyl-L-homocysteine + reumycin | - |
? | |
S-adenosyl-L-methionine + reumycin | - |
Burkholderia glumae | S-adenosyl-L-homocysteine + toxoflavin | - |
? | |
S-adenosyl-L-methionine + reumycin | the enzyme is a dual-specificity methyltransferase that catalyses the last two steps of toxoflavin biosynthesis. Toxoflavin is a major virulence factor of several bacterial crop pathogens | Burkholderia glumae | S-adenosyl-L-homocysteine + toxoflavin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
toxA | - |
Burkholderia glumae |
General Information | Comment | Organism |
---|---|---|
malfunction | toxA knockouts of Burkholderia glumae are less virulent in plant infection models | Burkholderia glumae |
metabolism | the enzyme is a dual-specificity methyltransferase that catalyses the last two steps of toxoflavin biosynthesis. Toxoflavin is a major virulence factor of several bacterial crop pathogens | Burkholderia glumae |