Literature summary for 2.1.1.348 extracted from

Liu, J.; Yue, Y.; Han, D.; Wang, X.; Fu, Y.; Zhang, L.; Jia, G.; Yu, M.; Lu, Z.; Deng, X.; Dai, Q.; Chen, W.; He, C.
A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation (2014), Nat. Chem. Biol., 10, 93-95 .

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Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q86U44 and Q9HCE5	Q86U44 i.e. catalytic subunit METTL3, Q9HCE5 i.e non-catalytic subunit METTL14	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
HEK-293FT cell	-	Homo sapiens	-
HeLa cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	both METTL3 and METTL14 preferentially methylate RNA substrates containing the consensus sequence GGACU. RNA probes containing consensus sequence GGAUU show a 10- to 60fold decrease in methylation. The combination of METTL3/METTL14 exhibits slightly higher activity towards probe with sequence GGACU in the stem than probe with sequence GGACU in the loop	Homo sapiens	?	-	?
S-adenosyl-L-methionine + adenine in mRNA	-	Homo sapiens	S-adenosyl-L-homocysteine + N6-methyladenine in mRNA	-	?

General Information

General Information	Comment	Organism
physiological function	METTL14 and m6A methyltransferase METTL3 form a stable heterodimer core complex of METTL3-14 that functions in cellular m6A deposition on mammalian nuclear RNAs. WTAP, a mammalian splicing factor, can interact with this complex and affect this methylation. Knockdown of cellular METTL3, METTL14, and WTAP decreases the m6A level in polyadenylated RNA by about 30%, 40%, and 50% in HeLa cells, respectively, and about 20%, 35%, and 42% in HEK-293FT cells, respectively	Homo sapiens

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Release 2023.1 (January 2023)