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Literature summary for 2.1.1.341 extracted from

  • Kohler, A.C.; Mills, M.J.L.; Adams, P.D.; Simmons, B.A.; Sale, K.L.
    Structure of aryl O-demethylase offers molecular insight into a catalytic tyrosine-dependent mechanism (2017), Proc. Natl. Acad. Sci. USA, 114, E3205-E3214.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.341

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.81 A resolution, structure reveals a unique demethylase fold and a canonical folate-binding domain Sphingomonas paucimobilis

Protein Variants

Protein Variants Comment Organism
H60A 97-99% loss of activity Sphingomonas paucimobilis
M61A 97-99% loss of activity Sphingomonas paucimobilis
R122A 97-99% loss of activity Sphingomonas paucimobilis
R122A/R147A complete loss of activity Sphingomonas paucimobilis
Y247F almost complete loss of activity, no discernible effect on overall protein fold or solution behavior Sphingomonas paucimobilis
Y29A 97-99% loss of activity Sphingomonas paucimobilis
Y31A 97-99% loss of activity Sphingomonas paucimobilis

Inhibitors

Inhibitors Comment Organism Structure
5-methyltetrahydrofolate competitive with respect to tetrahydrofolate, noncompetitive with respect to vanillate Sphingomonas paucimobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.63
-
 vanillate pH 8.0, 30°C Sphingomonas paucimobilis
0.72
-
 tetrahydrofolate pH 8.0, 30°C Sphingomonas paucimobilis

Organism

Organism UniProt Comment Textmining
Sphingomonas paucimobilis Q60FX1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme uses an ordered sequential mechanism Sphingomonas paucimobilis ?
-
 ?
vanillate + tetrahydrofolate
-
 Sphingomonas paucimobilis protocatechuate + 5-methyltetrahydrofolate
-
 ?

Synonyms

Synonyms Comment Organism
LigM
-
 Sphingomonas paucimobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Sphingomonas paucimobilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40
-
 Sphingomonas paucimobilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 inactive above Sphingomonas paucimobilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.76
-
 vanillate pH 8.0, 30°C Sphingomonas paucimobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Sphingomonas paucimobilis

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
 Sphingomonas paucimobilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
 5-methyltetrahydrofolate substrate vanillate, pH 8.0, 30°C Sphingomonas paucimobilis
0.17
-
 5-methyltetrahydrofolate substrate tetrahydrofolate, pH 8.0, 30°C Sphingomonas paucimobilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8
-
 tetrahydrofolate pH 8.0, 30°C Sphingomonas paucimobilis
9.14
-
 vanillate pH 8.0, 30°C Sphingomonas paucimobilis