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Literature summary for 2.1.1.341 extracted from

  • Harada, A.; Kamimura, N.; Takeuchi, K.; Yu, H.Y.; Masai, E.; Senda, T.
    The crystal structure of a new O-demethylase from Sphingobium sp. strain SYK-6 (2017), FEBS J., 284, 1855-1867.
    View publication on PubMed
Search for more literature for 2.1.1.341

Cloned(Commentary)

Cloned (Comment) Organism
-
 Sphingobium sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of apo form and in complex with substrates and tetrahydrofolate. Residues His60 and Tyr247 are essential to the catalytic activity of LigM. Their interactions with the oxygen atom in the methoxy group of vanillate seem to facilitate a methyl moiety transfer to tetrahydrofolate Sphingobium sp.

Protein Variants

Protein Variants Comment Organism
H60A loss of catalytic activity, mutant is able to bind both substrates Sphingobium sp.
Y247F loss of catalytic activity, mutant is able to bind both substrates Sphingobium sp.
Y31A loss of catalytic activity Sphingobium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.078
-
 vanillate wild-type, pH 8.0, 30°C Sphingobium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52300
-
-
 Sphingobium sp.
104000
-
 gel filtration Sphingobium sp.

Organism

Organism UniProt Comment Textmining
Sphingobium sp. G2IQS7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
vanillate + tetrahydrofolate
-
 Sphingobium sp. protocatechuate + 5-methyltetrahydrofolate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 52300, calculated Sphingobium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.385
-
 vanillate wild-type, pH 8.0, 30°C Sphingobium sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.5
-
-
 Sphingobium sp.