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Literature summary for 2.1.1.341 extracted from

  • Abe, T.; Masai, E.; Miyauchi, K.; Katayama, Y.; Fukuda, M.
    A tetrahydrofolate-dependent O-demethylase, LigM, is crucial for catabolism of vanillate and syringate in Sphingomonas paucimobilis SYK-6 (2005), J. Bacteriol., 187, 2030-2037.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
tetrahydrofolate dependent on Sphingomonas paucimobilis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sphingomonas paucimobilis
gene ligM, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strains JM109 and BL21(DE3) Sphingomonas paucimobilis

Protein Variants

Protein Variants Comment Organism
additional information disruption of ligM leads to significant growth retardation on both vanillate and syringate, a ligM desA double mutant completely looses the ability to transform vanillate, phenotypes, overview Sphingomonas paucimobilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
vanillate + tetrahydrofolate Sphingomonas paucimobilis
-
protocatechuate + 5-methyltetrahydrofolate
-
?
vanillate + tetrahydrofolate Sphingomonas paucimobilis
-
protocatechuate + 5-methyltetrahydrofolate procatechuic acid is 3,4-dihydroxybenzoate, which is further degraded via the procatechiuc acid 4,5-cleavage pathway ?
vanillate + tetrahydrofolate Sphingomonas paucimobilis SYK-6
-
protocatechuate + 5-methyltetrahydrofolate
-
?
vanillate + tetrahydrofolate Sphingomonas paucimobilis SYK-6
-
protocatechuate + 5-methyltetrahydrofolate procatechuic acid is 3,4-dihydroxybenzoate, which is further degraded via the procatechiuc acid 4,5-cleavage pathway ?

Organism

Organism UniProt Comment Textmining
Sphingomonas paucimobilis Q60FX1
-
-
Sphingomonas paucimobilis Q60FX1 gene ligM
-
Sphingomonas paucimobilis SYK-6 Q60FX1
-
-
Sphingomonas paucimobilis SYK-6 Q60FX1 gene ligM
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-O-methylgallate + tetrahydrofolate
-
Sphingomonas paucimobilis gallate + 5-methyltetrahydrofolate
-
?
additional information syringate is no substrate Sphingomonas paucimobilis ?
-
?
additional information no substrate: syringate Sphingomonas paucimobilis ?
-
?
additional information syringate is no substrate Sphingomonas paucimobilis SYK-6 ?
-
?
additional information no substrate: syringate Sphingomonas paucimobilis SYK-6 ?
-
?
vanillate + tetrahydrofolate
-
Sphingomonas paucimobilis protocatechuate + 5-methyltetrahydrofolate
-
?
vanillate + tetrahydrofolate
-
Sphingomonas paucimobilis protocatechuate + 5-methyltetrahydrofolate procatechuic acid is 3,4-dihydroxybenzoate ?
vanillate + tetrahydrofolate
-
Sphingomonas paucimobilis protocatechuate + 5-methyltetrahydrofolate procatechuic acid is 3,4-dihydroxybenzoate, which is further degraded via the procatechiuc acid 4,5-cleavage pathway ?
vanillate + tetrahydrofolate
-
Sphingomonas paucimobilis SYK-6 protocatechuate + 5-methyltetrahydrofolate
-
?
vanillate + tetrahydrofolate
-
Sphingomonas paucimobilis SYK-6 protocatechuate + 5-methyltetrahydrofolate procatechuic acid is 3,4-dihydroxybenzoate ?
vanillate + tetrahydrofolate
-
Sphingomonas paucimobilis SYK-6 protocatechuate + 5-methyltetrahydrofolate procatechuic acid is 3,4-dihydroxybenzoate, which is further degraded via the procatechiuc acid 4,5-cleavage pathway ?

Synonyms

Synonyms Comment Organism
LigM
-
Sphingomonas paucimobilis
tetrahydrofolate-dependent O-demethylase
-
Sphingomonas paucimobilis
vanillate/3-O-methylgallate O-demethylase
-
Sphingomonas paucimobilis
vanillate/3MGA O-demethylase
-
Sphingomonas paucimobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Sphingomonas paucimobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Sphingomonas paucimobilis

Cofactor

Cofactor Comment Organism Structure
NADH
-
Sphingomonas paucimobilis

General Information

General Information Comment Organism
physiological function disruption of LigM leads to significant growth retardation on both vanillate and syringate. The ability of the mutant to transform vanillate is markedly decreased, and it completely lost the 3-O-methylgallate O-demethylase activity. A LigM syringate demethylase DesA double mutant completely lost the ability to transform vanillate Sphingomonas paucimobilis