Inhibitors | Comment | Organism | Structure |
---|---|---|---|
potassium phosphate | - |
Hordeum vulgare | |
sodium carbonate | - |
Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
1 * 43000, SDS-PAGE | Hordeum vulgare |
45000 | - |
gel filtration | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | first leaves synthesize gramine constitutively | Hordeum vulgare | - |
additional information | no activity in seedling | Hordeum vulgare | - |
Storage Stability | Organism |
---|---|
freeze-thawing reduces activity by 50% | Hordeum vulgare |
leaf extract, 4°C, 75% residual activity after 4 days | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + 3-aminomethylindole | - |
Hordeum vulgare | 2 S-adenosyl-L-homocysteine + gramine | overall reaction | ? | |
2 S-adenosyl-L-methionine + N-methyl-3-aminomethylindole | - |
Hordeum vulgare | 2 S-adenosyl-L-homocysteine + (1H-indol-3-yl)-N,N-dimethylmethanamine | - |
? | |
additional information | no substrate: tryptamine. Poor substrate: tyramine | Hordeum vulgare | ? | - |
? | |
S-adenosyl-L-methionine + 3-aminomethylindole | - |
Hordeum vulgare | S-adenosyl-L-homocysteine + N-methyl-3-aminomethylindole | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000, SDS-PAGE | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Hordeum vulgare |
General Information | Comment | Organism |
---|---|---|
physiological function | activity in expanding fifth leaves is increased 8- to 20fold by growth at high temperatures(35°C day/30°C night) compared to cool temperatures (15°C/10°C). Several days of high temperature are required for full induction of activity. No induction occurs in leaves which completed expansion in cool conditions before exposure to high temperature | Hordeum vulgare |