Literature summary for 2.1.1.338 extracted from

Nagel, J.; Culley, L.; Lu, Y.; Liu, E.; Matthews, P.; Stevens, J.; Page, J.
EST analysis of hop glandular trichomes identifies an O-methyltransferase that catalyzes the biosynthesis of xanthohumol (2008), Plant Cell, 20, 186-200.

Search for more literature for 2.1.1.338

Inhibitors

Inhibitors	Comment	Organism	Structure
desmethylxanthohumol	competitive with respect to xanthohumol	Humulus lupulus
S-adenosyl-L-homocysteine	-	Humulus lupulus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.018	-	desmethylxanthohumol	pH 7.5, 30°C	Humulus lupulus
0.286	-	S-adenosyl-L-methionine	pH 7.5, 30°C	Humulus lupulus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	bivalent cations (Mg2+, Fe2+, Zn2+, Cu2+, Ca2+, and Co2+) are not required for activity	Humulus lupulus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39265	-	-	Humulus lupulus
76000	-	gel filtration	Humulus lupulus

Organism

Organism	UniProt	Comment	Textmining
Humulus lupulus	B0ZB55	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + caffeic acid	-	Humulus lupulus	S-adenosyl-L-homocysteine + ?	7% of the activity with desmethylxanthohumol	?
S-adenosyl-L-methionine + desmethylxanthohumol	-	Humulus lupulus	S-adenosyl-L-homocysteine + xanthohumol	-	?
S-adenosyl-L-methionine + xanthogalenol	445% of the activity with desmethylxanthohumol	Humulus lupulus	S-adenosyl-L-homocysteine + ?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 39265, calculated	Humulus lupulus

Synonyms

Synonyms	Comment	Organism
OMT1	-	Humulus lupulus
S-adenosyl-L-methionine-dependent O-methyltransferase	-	Humulus lupulus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	37	-	Humulus lupulus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Humulus lupulus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.002	-	desmethylxanthohumol	pH 7.5, 30°C	Humulus lupulus
0.098	-	S-adenosyl-L-homocysteine	pH 7.5, 30°C	Humulus lupulus

General Information

General Information	Comment	Organism
physiological function	OMT1 performs the final reaction in xanthohumol biosynthesis by methylating desmethylxanthohumol to form xanthohumol	Humulus lupulus

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Release 2026.1 (March 2026)