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Literature summary for 2.1.1.337 extracted from

  • Morris, J.S.; Facchini, P.J.
    Isolation and characterization of reticuline N-methyltransferase involved in biosynthesis of the aporphine alkaloid magnoflorine in opium poppy (2016), J. Biol. Chem., 291, 23416-23427.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Papaver somniferum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
(S)-reticuline pH 7.0, 30°C Papaver somniferum
0.085
-
(R)-reticuline pH 7.0, 30°C Papaver somniferum
0.168
-
S-adenosyl-L-methionine pH 7.0, 30°C Papaver somniferum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41900
-
-
Papaver somniferum
45000
-
-
Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Papaver somniferum RNMT shows a strong preference for substrates with a tertiary amine, although alkaloids containing a secondary amine (e.g. (R,S)-tetrahydropapaverine, (S)-coclaurine) are also accepted as substrates. In the case of substrates with secondary amines, RNMT catalyzes two sequential N-methylations ?
-
?
S-adenosyl-L-methionine + (+)-bulbocapnine Papaver somniferum 75% substrate conversion S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + (+)-glaucine Papaver somniferum 47% substrate conversion S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + (R)-reticuline Papaver somniferum 98% substrate conversion S-adenosyl-L-homocysteine + (S)-tembetarine
-
?
S-adenosyl-L-methionine + (S)-corytuberine Papaver somniferum 82% substrate conversion S-adenosyl-L-homocysteine + magnoflorine
-
?
S-adenosyl-L-methionine + (S)-reticuline Papaver somniferum 47% substrate conversion S-adenosyl-L-homocysteine + (S)-tembetarine
-
?

Organism

Organism UniProt Comment Textmining
Papaver somniferum A0A1C9U5X5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root highest transcript level Papaver somniferum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information RNMT shows a strong preference for substrates with a tertiary amine, although alkaloids containing a secondary amine (e.g. (R,S)-tetrahydropapaverine, (S)-coclaurine) are also accepted as substrates. In the case of substrates with secondary amines, RNMT catalyzes two sequential N-methylations Papaver somniferum ?
-
?
additional information RNMT shows a strong preference for substrates with a tertiary amine, although alkaloids containing a secondary amine (e.g. (R,S)-tetrahydropapaverine) are also accepted as substrates. In the case of substrates with secondary amines, RNMT catalyzes two sequential N-methylations Papaver somniferum ?
-
?
S-adenosyl-L-methionine + (+)-bulbocapnine 75% substrate conversion Papaver somniferum S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + (+)-glaucine 47% substrate conversion Papaver somniferum S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + (R)-reticuline 98% substrate conversion Papaver somniferum S-adenosyl-L-homocysteine + (S)-tembetarine
-
?
S-adenosyl-L-methionine + (R,S)-tetrahydropapaverine 24% substrate conversion Papaver somniferum S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + (S)-corytuberine 82% substrate conversion Papaver somniferum S-adenosyl-L-homocysteine + magnoflorine
-
?
S-adenosyl-L-methionine + (S)-reticuline 47% substrate conversion Papaver somniferum S-adenosyl-L-homocysteine + (S)-tembetarine
-
?

Subunits

Subunits Comment Organism
? x * 41900, calculated, x * 45000, SDS-PAGE Papaver somniferum

Synonyms

Synonyms Comment Organism
NMT3
-
Papaver somniferum
RNMT
-
Papaver somniferum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Papaver somniferum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.028
-
(S)-reticuline pH 7.0, 30°C Papaver somniferum
0.047
-
S-adenosyl-L-methionine pH 7.0, 30°C Papaver somniferum
0.053
-
(R)-reticuline pH 7.0, 30°C Papaver somniferum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9 substrate (S)-reticuline Papaver somniferum
8.5
-
substrate (R)-reticuline Papaver somniferum

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Papaver somniferum

pI Value

Organism Comment pI Value Maximum pI Value
Papaver somniferum calculated
-
5.9

General Information

General Information Comment Organism
physiological function suppression of the gene encoding reticuline N-methyltransferase results in a significant decrease in magnoflorine accumulation and a concomitant increase in corytuberine levels in roots Papaver somniferum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.28
-
S-adenosyl-L-methionine pH 7.0, 30°C Papaver somniferum
0.62
-
(R)-reticuline pH 7.0, 30°C Papaver somniferum
0.65
-
(S)-reticuline pH 7.0, 30°C Papaver somniferum