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Literature summary for 2.1.1.336 extracted from
- Molecular Cloning and Characterization of a meta/para-O-Methyltransferase from Lycoris aurea (2018), Int. J. Mol. Sci., 19, 1911 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologous expression in Escherichia coli BL21 (DE3) | Lycoris aurea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02032 | - |
caffeic acid | pH 7.8, 37°C | Lycoris aurea |  |
| 0.15194 | - |
3,4-Dihydroxybenzaldehyde | pH 7.8, 37°C | Lycoris aurea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | the enzyme is mainly localized in cytoplasm and endosome | Lycoris aurea | 5737 | - |
| endosome | the enzyme is mainly localized in cytoplasm and endosome | Lycoris aurea | 5768 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 0.05-0.1 mM, activates | Lycoris aurea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26980 | - |
calculated from sequence | Lycoris aurea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + norbelladine | Lycoris aurea | 4'-O-methylnorbelladine is an universal precursor of all the primary Amaryllidaceae alkaloid skeletons | S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lycoris aurea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lycoris aurea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bulb | - |
Lycoris aurea | - |
| column | - |
Lycoris aurea | - |
| filament | - |
Lycoris aurea | - |
| flower stalk | - |
Lycoris aurea | - |
| leaf | - |
Lycoris aurea | - |
| petal | - |
Lycoris aurea | - |
| plant ovary | highest expression level | Lycoris aurea | - |
| root | - |
Lycoris aurea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde | - |
Lycoris aurea | 2 S-adenosyl-L-homocysteine + vanillin + isovanillin | - |
? | |
| 2 S-adenosyl-L-methionine + caffeic acid | - |
Lycoris aurea | 2 S-adenosyl-L-homocysteine + ferulic acid + isoferulic acid | - |
? | |
| 2 S-adenosyl-L-methionine + norbelladine | - |
Lycoris aurea | 2 S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine + 3'-O-methylnorbelladine | - |
? | |
| additional information | no activity with vanillin, isovanillin, o-vanillin or tyramine | Lycoris aurea | ? | - |
- |
|
| S-adenosyl-L-methionine + norbelladine | 4'-O-methylnorbelladine is an universal precursor of all the primary Amaryllidaceae alkaloid skeletons | Lycoris aurea | S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LaOMT1 | - |
Lycoris aurea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 45 | - |
Lycoris aurea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | 30°C: about 60% of maximal activity, 50°C: about 55% of maximal activity | Lycoris aurea |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00038 | - |
caffeic acid | pH 7.8, 37°C | Lycoris aurea | |
| 0.0173 | - |
3,4-Dihydroxybenzaldehyde | pH 7.8, 37°C | Lycoris aurea | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Lycoris aurea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | pH 6.5: about 35% of maximal activity, pH 9.3: about 90% of maximal activity | Lycoris aurea |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Lycoris aurea | calculated from sequence | - |
5 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Lycoris aurea | abscisic acid treatment dramatically decreases the expression level | down |
| Lycoris aurea | sodium chloride, cold, polyethylene glycol, sodium nitroprusside, and methyl jasmonate treatments can significantly increase LaOMT1 transcripts | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme may play a multifunctional role, and could be the foundation for Amaryllidaceae alkaloid biosynthesis in Lycoris aurea | Lycoris aurea |
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