Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Codon Plus RIL cells | Narcissus aff. pseudonarcissus MK-2014 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
norbelladine | at pH 8.8 and 37°C | Narcissus aff. pseudonarcissus MK-2014 | |
0.0019 | - |
N-methylnorbelladine | at pH 8.8 and 37°C | Narcissus aff. pseudonarcissus MK-2014 | |
0.0073 | - |
dopamine | at pH 8.8 and 37°C | Narcissus aff. pseudonarcissus MK-2014 | |
0.0285 | - |
S-adenosyl-L-methionine | at pH 8.8 and 37°C | Narcissus aff. pseudonarcissus MK-2014 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, calculated from amino acid sequence | Narcissus aff. pseudonarcissus MK-2014 |
29000 | - |
- |
Narcissus aff. pseudonarcissus MK-2014 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + norbelladine | Narcissus aff. pseudonarcissus MK-2014 | - |
S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Narcissus aff. pseudonarcissus MK-2014 | A0A077EWA5 | - |
- |
Purification (Comment) | Organism |
---|---|
Talon resin column chromatography | Narcissus aff. pseudonarcissus MK-2014 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bulb | - |
Narcissus aff. pseudonarcissus MK-2014 | - |
inflorescence | - |
Narcissus aff. pseudonarcissus MK-2014 | - |
leaf | - |
Narcissus aff. pseudonarcissus MK-2014 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with caffeic acid, vanillin, 3,4-dihydroxybenzaldehyde, and tyramine | Narcissus aff. pseudonarcissus MK-2014 | ? | - |
? | |
S-adenosyl-L-methionine + dopamine | - |
Narcissus aff. pseudonarcissus MK-2014 | ? | - |
? | |
S-adenosyl-L-methionine + N-methylnorbelladine | - |
Narcissus aff. pseudonarcissus MK-2014 | ? | - |
? | |
S-adenosyl-L-methionine + norbelladine | - |
Narcissus aff. pseudonarcissus MK-2014 | S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, calculated from amino acid sequence | Narcissus aff. pseudonarcissus MK-2014 |
? | x * 29000, His-tagged enzyme, SDS-PAGE | Narcissus aff. pseudonarcissus MK-2014 |
Synonyms | Comment | Organism |
---|---|---|
N4OMT1 | - |
Narcissus aff. pseudonarcissus MK-2014 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Narcissus aff. pseudonarcissus MK-2014 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
norbelladine | at pH 8.8 and 37°C | Narcissus aff. pseudonarcissus MK-2014 | |
0.043 | - |
N-methylnorbelladine | at pH 8.8 and 37°C | Narcissus aff. pseudonarcissus MK-2014 | |
0.06 | - |
dopamine | at pH 8.8 and 37°C | Narcissus aff. pseudonarcissus MK-2014 | |
0.075 | - |
S-adenosyl-L-methionine | at pH 8.8 and 37°C | Narcissus aff. pseudonarcissus MK-2014 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Narcissus aff. pseudonarcissus MK-2014 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
S-adenosyl-L-methionine | at pH 8.8 and 37°C | Narcissus aff. pseudonarcissus MK-2014 | |
8.3 | - |
dopamine | at pH 8.8 and 37°C | Narcissus aff. pseudonarcissus MK-2014 | |
13.3 | - |
norbelladine | at pH 8.8 and 37°C | Narcissus aff. pseudonarcissus MK-2014 | |
21.7 | - |
N-methylnorbelladine | at pH 8.8 and 37°C | Narcissus aff. pseudonarcissus MK-2014 |