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Literature summary for 2.1.1.336 extracted from

  • Kilgore, M.B.; Augustin, M.M.; Starks, C.M.; ONeil-Johnson, M.; May, G.D.; Crow, J.A.; Kutchan, T.M.
    Cloning and characterization of a norbelladine 4-O-methyltransferase involved in the biosynthesis of the Alzheimers drug galanthamine in Narcissus sp. aff. pseudonarcissus (2014), PLoS ONE, 9, e103223.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Codon Plus RIL cells Narcissus aff. pseudonarcissus MK-2014

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
norbelladine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
0.0019
-
N-methylnorbelladine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
0.0073
-
dopamine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
0.0285
-
S-adenosyl-L-methionine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
x * 27000, calculated from amino acid sequence Narcissus aff. pseudonarcissus MK-2014
29000
-
-
Narcissus aff. pseudonarcissus MK-2014

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + norbelladine Narcissus aff. pseudonarcissus MK-2014
-
S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine
-
?

Organism

Organism UniProt Comment Textmining
Narcissus aff. pseudonarcissus MK-2014 A0A077EWA5
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon resin column chromatography Narcissus aff. pseudonarcissus MK-2014

Source Tissue

Source Tissue Comment Organism Textmining
bulb
-
Narcissus aff. pseudonarcissus MK-2014
-
inflorescence
-
Narcissus aff. pseudonarcissus MK-2014
-
leaf
-
Narcissus aff. pseudonarcissus MK-2014
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with caffeic acid, vanillin, 3,4-dihydroxybenzaldehyde, and tyramine Narcissus aff. pseudonarcissus MK-2014 ?
-
?
S-adenosyl-L-methionine + dopamine
-
Narcissus aff. pseudonarcissus MK-2014 ?
-
?
S-adenosyl-L-methionine + N-methylnorbelladine
-
Narcissus aff. pseudonarcissus MK-2014 ?
-
?
S-adenosyl-L-methionine + norbelladine
-
Narcissus aff. pseudonarcissus MK-2014 S-adenosyl-L-homocysteine + 4'-O-methylnorbelladine
-
?

Subunits

Subunits Comment Organism
? x * 27000, calculated from amino acid sequence Narcissus aff. pseudonarcissus MK-2014
? x * 29000, His-tagged enzyme, SDS-PAGE Narcissus aff. pseudonarcissus MK-2014

Synonyms

Synonyms Comment Organism
N4OMT1
-
Narcissus aff. pseudonarcissus MK-2014

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Narcissus aff. pseudonarcissus MK-2014

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.022
-
norbelladine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
0.043
-
N-methylnorbelladine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
0.06
-
dopamine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
0.075
-
S-adenosyl-L-methionine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
Narcissus aff. pseudonarcissus MK-2014

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7
-
S-adenosyl-L-methionine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
8.3
-
dopamine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
13.3
-
norbelladine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014
21.7
-
N-methylnorbelladine at pH 8.8 and 37°C Narcissus aff. pseudonarcissus MK-2014