Crystallization (Comment) | Organism |
---|---|
a truncated protein lacking the first 32 residues of the N-terminus but with an additional His6 tag at the C-terminus is crystallized by hanging-drop vapour-diffusion method using 15% (w/v) PEG 3350 and 150 mM MgSO4, at 10°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27307 | - |
x * 27307, calculated from sequence | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine46 in tRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A8I5 | strain K12 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine46 in tRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27307, calculated from sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
transfer RNA (m7G46) methyltransferase | - |
Escherichia coli |
TrmB | TrmB belongs to the Rossmann-fold methyltransferase family | Escherichia coli |
tRNA (m7G46) methyltransferase | - |
Escherichia coli |