Protein Variants | Comment | Organism |
---|---|---|
additional information | strains lacking Trm8p/Trm82p tRNA m7G methyltransferase, are temperature-sensitive in synthetic media containing glycerol, trm82-delta strain shows reduced levels of Trm8p, but not of its mRNA, the growth phenotype of trm8 mutants correlates with lack of tRNA m7G methyltransferase activity in vitro and in vivo, bacterial Trm8p orthologs complement the trm8-delta trm82-delta growth phenotype and restore m7G-methyltransferase activity in vitro and in vivo | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine46 in tRNA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q03774 and Q12009 | Q03774: subunit Trm82, Q2009: subunit Trm8 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine46 in tRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | Trm8p is required for m7G modification. Trm82p is required to maintain cellular levels of Trm8p and to stabilize Trm8p in an active conformation | Saccharomyces cerevisiae |