Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
by hanging drop method, at a resolution of 2.1 A, Rossmann-fold methyltransferase structure with the N-terminal helix folded on the opposite site of the catalytic domain | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant strain, trmB gene inactivated by an insertion, shows normal growth | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | contains three potassium ions per asymmetric unit, one in the interface between monomers and one in a region involved in AdoMet binding in the homologous catechol O-MTase, bound to the main chain carbonyls of residues Asn115 and Gly46 | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O34522 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Subunits | Comment | Organism |
---|---|---|
dimer | crystallographic studies, gel filtration, dimeric both in crystal and in solution | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
TrmB | - |
Bacillus subtilis |
tRNA (m7G46) methyltransferase | - |
Bacillus subtilis |