Literature summary for 2.1.1.33 extracted from

Matsumoto, K.; Toyooka, T.; Tomikawa, C.; Ochi, A.; Takano, Y.; Takayanagi, N.; Endo, Y.; Hori, H.
RNA recognition mechanism of eukaryote tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex) (2007), FEBS Lett., 581, 1599-1604.

Search for more literature for 2.1.1.33

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00025	-	guanine46 in tRNA	pH 7.5, 30°C, tRNA variant with substitution of C48 by A	Saccharomyces cerevisiae
0.00035	-	guanine46 in tRNA	pH 7.5, 30°C, tRNA variant with disruption of tertiary base pairs between D- and T-arms	Saccharomyces cerevisiae
0.0005	-	guanine46 in tRNA	pH 7.5, 30°C, tRNA variant with disruption of C13-G22-G46 tertiary base pair	Saccharomyces cerevisiae
0.0014	-	guanine46 in tRNA	pH 7.5, 30°C, tRNA variant with deletion of the anticodon-arm	Saccharomyces cerevisiae
0.0015	-	guanine46 in tRNA	pH 7.5, 30°C, RNA	Saccharomyces cerevisiae
0.0037	-	guanine46 in tRNA	pH 7.5, 30°C, tRNA variant with deletion of the aminoacyl-stem	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + guanine46 in tRNA	Saccharomyces cerevisiae	-	S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	Q03774 and Q12009	Q03774: subunit Trm82, Q2009: subunit Trm8	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanine46 in tRNA	-	Saccharomyces cerevisiae	S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA	-	?

Subunits

Subunits	Comment	Organism
heterodimer	the enzyme contains two protein subunits: TRm8 and TRm82	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
Trm8Trm82 complex	-	Saccharomyces cerevisiae
tRNA (m7G46) methyltransferase	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)