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Literature summary for 2.1.1.33 extracted from

  • Alexandrov, A.; Martzen, M.R.; Phizicky, E.M.
    Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA (2002), RNA, 8, 1253-1266.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of both METTL1 and WDR4 in Saccharomyces cerevisiae Homo sapiens
ORF YDL201w encodes Trm8 and ORF YDR165w encodes Trm82, both proteins form a complex with m7G-methyltransferase activity, Trm8 contains a binding domain for S-adenosyl-L-methionine: GXGXG, coexpression of Trm8/Trm82 in Escherichia coli BL21 Codon Plus (DE3) as His6-fusion proteins under control of the Ptac-promoter Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information strains lacking either Trm8 or Trm82 yield extracts with no detectable enzyme activity, and have severely reduced m7G-modified tRNA in vivo Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine46 in tRNA Saccharomyces cerevisiae complex of 2 proteins Trm8 and Trm82 is required for efficient m7G-methyltransferase activity in vitro and in vivo, tRNA site G46 is methylated in vivo S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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Organism

Organism UniProt Comment Textmining
Homo sapiens
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Saccharomyces cerevisiae
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strains BY4743, 33523, 33899 and YM317
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Purification (Commentary)

Purification (Comment) Organism
copurification of 2 proteins, Trm8 and Trm82 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information complex of 2 proteins Trm8 and Trm82 is required for efficient m7G-methyltransferase activity in vitro and in vivo, but Trm82 is not an absolute requirement for activity in vitro, Trm8 may be the catalytically active subunit Saccharomyces cerevisiae additional information
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additional information proteins METTL1 and WDR4 are required for m7G-methyltransferase activity Homo sapiens additional information
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S-adenosyl-L-methionine + guanine46 in tRNA methyl group acceptors: tRNACys, tRNATrp, tRNAPro, tRNAMet, tRNAMet-i and 2 of each tRNAPhe, tRNAVal and tRNALys, methylation at G46 in the extra loop Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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S-adenosyl-L-methionine + guanine46 in tRNA methyl group acceptor: yeast pre-tRNAPhe Homo sapiens S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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S-adenosyl-L-methionine + guanine46 in tRNA methyl group acceptor: yeast pre-tRNAPhe Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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?
S-adenosyl-L-methionine + guanine46 in tRNA complex of 2 proteins Trm8 and Trm82 is required for efficient m7G-methyltransferase activity in vitro and in vivo, tRNA site G46 is methylated in vivo Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
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assay at Saccharomyces cerevisiae
37
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assay at Homo sapiens
37
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30fold higher activity when assayed at 37°C than at 30°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
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Saccharomyces cerevisiae