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Literature summary for 2.1.1.324 extracted from
- In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa (2008), J. Am. Chem. Soc., 130, 4954-4967.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Saccharopolyspora spinosa |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 2 mM used in assay conditions | Saccharopolyspora spinosa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
2 * 28000, SDS-PAGE | Saccharopolyspora spinosa |
| 28730 | - |
2 * 28730, calculated from amino acid sequence | Saccharopolyspora spinosa |
| 61200 | - |
gel filtration | Saccharopolyspora spinosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose | Saccharopolyspora spinosa | - |
S-adenosyl-L-homocysteine + dTDP-alpha-D-forosamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharopolyspora spinosa | Q9ALP0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Sephacryl S-200 gel filtration | Saccharopolyspora spinosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose | - |
Saccharopolyspora spinosa | S-adenosyl-L-homocysteine + dTDP-alpha-D-forosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 28000, SDS-PAGE | Saccharopolyspora spinosa |
| homodimer | 2 * 28730, calculated from amino acid sequence | Saccharopolyspora spinosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SpnS | - |
Saccharopolyspora spinosa |
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Release 2023.1 (January 2023)
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