Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in a structural model, the carboxylate group of the position 4 Asp258 points away from the substrate arginine, possibly to coordinate the binding of one or both of the two lysine residues adjacent to the methylated arginine in the FKRKY sequence of substrate SF3B2 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D258G | mutation mimics type I enzymes, complete loss of activity | Homo sapiens |
G260E | mutation mimics isoform PRMT7, complete loss of activity | Homo sapiens |
additional information | construction of deletion mutant, in which the TPR motif or the N terminus is missing, and two C-terminal mutants. No activity is observed with any of the mutants | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6P2P2 | isoform PRMT9 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + [splicing factor SF3B2]-L-arginine | - |
Homo sapiens | 2 S-adenosyl-L-homocysteine + [splicing factor SF3B2]-Nomega,Nomega'-dimethyl-L-arginine | overall reaction, isoform PRMT9 symmetrically dimethylates arginine residues on splicing factor SF3B2. A peptide containing the methylatable Arg508 of SF3B2 is not recognized by PRMT9 in vitro. Amino acid substitutions of residues surrounding Arg508 have no great effect on PRMT9 recognition of SF3B2, but moving the arginine residue within this sequence abolishes methylation | ? | |
additional information | isoform PRMT9 poorly methylates PRMT5 substrate GST-GAR | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PRMT9 | - |
Homo sapiens |