Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.320 extracted from

  • Hadjikyriacou, A.; Yang, Y.; Espejo, A.; Bedford, M.T.; Clarke, S.G.
    Unique features of human protein arginine methyltransferase 9 (PRMT9) and its substrate RNA splicing Factor SF3B2 (2015), J. Biol. Chem., 290, 16723-16743.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in a structural model, the carboxylate group of the position 4 Asp258 points away from the substrate arginine, possibly to coordinate the binding of one or both of the two lysine residues adjacent to the methylated arginine in the FKRKY sequence of substrate SF3B2 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D258G mutation mimics type I enzymes, complete loss of activity Homo sapiens
G260E mutation mimics isoform PRMT7, complete loss of activity Homo sapiens
additional information construction of deletion mutant, in which the TPR motif or the N terminus is missing, and two C-terminal mutants. No activity is observed with any of the mutants Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Homo sapiens 5737
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6P2P2 isoform PRMT9
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + [splicing factor SF3B2]-L-arginine
-
Homo sapiens 2 S-adenosyl-L-homocysteine + [splicing factor SF3B2]-Nomega,Nomega'-dimethyl-L-arginine overall reaction, isoform PRMT9 symmetrically dimethylates arginine residues on splicing factor SF3B2. A peptide containing the methylatable Arg508 of SF3B2 is not recognized by PRMT9 in vitro. Amino acid substitutions of residues surrounding Arg508 have no great effect on PRMT9 recognition of SF3B2, but moving the arginine residue within this sequence abolishes methylation ?
additional information isoform PRMT9 poorly methylates PRMT5 substrate GST-GAR Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
PRMT9
-
Homo sapiens