BRENDA - Enzyme Database
show all sequences of 2.1.1.304

Characterization of SfmD as a heme peroxidase that catalyzes the regioselective hydroxylation of 3-methyltyrosine to 3-hydroxy-5-methyltyrosine in saframycin A biosynthesis

Tang, M.C.; Fu, C.Y.; Tang, G.L.; J. Biol. Chem. 287, 5112-5121 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Streptomyces lavendulae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41800
-
x * 41800, SDS-PAGE
Streptomyces lavendulae
42000
-
x * 42000, SDS-PAGE
Streptomyces lavendulae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + L-tyrosine
Streptomyces lavendulae
the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family
S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
-
?
S-adenosyl-L-methionine + L-tyrosine
Streptomyces lavendulae NRRL 11002
the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family
S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces lavendulae
B0CN31
-
-
Streptomyces lavendulae NRRL 11002
B0CN31
-
-
Purification (Commentary)
Commentary
Organism
-
Streptomyces lavendulae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + L-tyrosine
-
725507
Streptomyces lavendulae
S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
-
-
?
S-adenosyl-L-methionine + L-tyrosine
the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family
725507
Streptomyces lavendulae
S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
-
-
?
S-adenosyl-L-methionine + L-tyrosine
-
725507
Streptomyces lavendulae NRRL 11002
S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
-
-
?
S-adenosyl-L-methionine + L-tyrosine
the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family
725507
Streptomyces lavendulae NRRL 11002
S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 41800, SDS-PAGE; x * 42000, SDS-PAGE
Streptomyces lavendulae
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Streptomyces lavendulae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41800
-
x * 41800, SDS-PAGE
Streptomyces lavendulae
42000
-
x * 42000, SDS-PAGE
Streptomyces lavendulae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-adenosyl-L-methionine + L-tyrosine
Streptomyces lavendulae
the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family
S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
-
?
S-adenosyl-L-methionine + L-tyrosine
Streptomyces lavendulae NRRL 11002
the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family
S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces lavendulae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + L-tyrosine
-
725507
Streptomyces lavendulae
S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
-
-
?
S-adenosyl-L-methionine + L-tyrosine
the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family
725507
Streptomyces lavendulae
S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
-
-
?
S-adenosyl-L-methionine + L-tyrosine
-
725507
Streptomyces lavendulae NRRL 11002
S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
-
-
?
S-adenosyl-L-methionine + L-tyrosine
the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family
725507
Streptomyces lavendulae NRRL 11002
S-adenosyl-L-homocysteine + 3-methyl-L-tyrosine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 41800, SDS-PAGE; x * 42000, SDS-PAGE
Streptomyces lavendulae
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family
Streptomyces lavendulae
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in biosynthesis of saframycin A, a potent antitumor antibiotic that belongs to the tetrahydroisoquinoline family
Streptomyces lavendulae
Other publictions for EC 2.1.1.304
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725507
Tang
Characterization of SfmD as a ...
Streptomyces lavendulae, Streptomyces lavendulae NRRL 11002
J. Biol. Chem.
287
5112-5121
2012
-
-
1
-
-
-
-
-
-
-
2
2
-
2
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725683
Fu
Biosynthesis of 3-hydroxy-5-me ...
Streptomyces lavendulae
J. Microbiol. Biotechnol.
19
439-446
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-