Cloned (Comment) | Organism |
---|---|
the enzyme is overproduced as an N-terminal His6-tagged fusion protein using expression plasmid pBS5039 in Escherichia coli BL21(DE3) | Streptomyces carzinostaticus |
Crystallization (Comment) | Organism |
---|---|
the enzyme is crystallized with a substituted naphthoic acid and/or S-adenosyl-L-methionine/S-adenosyl-L-homocysteine by the hanging drop vapor diffusion method | Streptomyces carzinostaticus |
Protein Variants | Comment | Organism |
---|---|---|
R11A | kcat/Km is 88% of the wild-type value | Streptomyces carzinostaticus |
R11K | kcat/Km is 110% of the wild-type value | Streptomyces carzinostaticus |
R11W | kcat/Km is 45% of the wild-type value | Streptomyces carzinostaticus |
Y293I | kcat/Km is 35% of the wild-type value | Streptomyces carzinostaticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.206 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, wild-type enzyme | Streptomyces carzinostaticus | |
0.319 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, mutant enzyme R11K | Streptomyces carzinostaticus | |
0.4 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, mutant enzyme R11W | Streptomyces carzinostaticus | |
0.419 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, mutant enzyme R11A | Streptomyces carzinostaticus | |
0.649 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, mutant enzyme Y293I | Streptomyces carzinostaticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 2,7-dihydroxy-5-methyl-1-naphthoate | Streptomyces carzinostaticus | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | S-adenosyl-L-homocysteine + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces carzinostaticus | Q84HC8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces carzinostaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 2,7-dihydroxy-5-methyl-1-naphthoate | - |
Streptomyces carzinostaticus | S-adenosyl-L-homocysteine + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | - |
? | |
S-adenosyl-L-methionine + 2,7-dihydroxy-5-methyl-1-naphthoate | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | Streptomyces carzinostaticus | S-adenosyl-L-homocysteine + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NcsB1 | - |
Streptomyces carzinostaticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptomyces carzinostaticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, mutant enzyme R11W | Streptomyces carzinostaticus | |
0.0115 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, wild-type enzyme | Streptomyces carzinostaticus | |
0.013 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, mutant enzyme Y293I | Streptomyces carzinostaticus | |
0.02 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, mutant enzyme R11A | Streptomyces carzinostaticus | |
0.02 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, mutant enzyme R11K | Streptomyces carzinostaticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptomyces carzinostaticus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin | Streptomyces carzinostaticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, mutant enzyme Y293I | Streptomyces carzinostaticus | |
0.025 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, mutant enzyme R11W | Streptomyces carzinostaticus | |
0.048 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, mutant enzyme R11A | Streptomyces carzinostaticus | |
0.06 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, wild-type enzyme | Streptomyces carzinostaticus | |
0.063 | - |
2,7-dihydroxy-5-methyl-1-naphthoate | pH 7.5, 25°C, mutant enzyme TR11K | Streptomyces carzinostaticus |