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show all sequences of 2.1.1.300

Purification, crystallization and X-ray diffraction analysis of pavine N-methyltransferase from Thalictrum flavum

Jain, A.; Ziegler, J.; Liscombe, D.K.; Facchini, P.J.; Tucker, P.A.; Panjikar, S.; Acta Crystallogr. Sect. F 64, 1066-1069 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
heterologously expressed in Escherichia coli
Thalictrum flavum
Crystallization (Commentary)
Crystallization
Organism
sitting-drop vapour-diffusion method, hanging-drop vapour-diffusion method at 16°C, crystallized in space group P2(1). The structure is solved at 2.0 A resolution using a xenon derivative and the single isomorphous replacement with anomalous scattering method
Thalictrum flavum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thalictrum flavum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Thalictrum flavum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + (+/-)-pavine
-
723837
Thalictrum flavum
S-adenosyl-L-homocysteine + N-methylpavine
-
-
-
?
S-adenosyl-L-methionine + (R,S)-stylopine
-
723837
Thalictrum flavum
S-adenosyl-L-homocysteine + N-methylstylopine
-
-
-
?
S-adenosyl-L-methionine + (S)-scoulerine
-
723837
Thalictrum flavum
S-adenosyl-L-homocysteine + N-methyl-(S)-scoulerine
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
heterologously expressed in Escherichia coli
Thalictrum flavum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting-drop vapour-diffusion method, hanging-drop vapour-diffusion method at 16°C, crystallized in space group P2(1). The structure is solved at 2.0 A resolution using a xenon derivative and the single isomorphous replacement with anomalous scattering method
Thalictrum flavum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thalictrum flavum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-adenosyl-L-methionine + (+/-)-pavine
-
723837
Thalictrum flavum
S-adenosyl-L-homocysteine + N-methylpavine
-
-
-
?
S-adenosyl-L-methionine + (R,S)-stylopine
-
723837
Thalictrum flavum
S-adenosyl-L-homocysteine + N-methylstylopine
-
-
-
?
S-adenosyl-L-methionine + (S)-scoulerine
-
723837
Thalictrum flavum
S-adenosyl-L-homocysteine + N-methyl-(S)-scoulerine
-
-
-
?
Other publictions for EC 2.1.1.300
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723837
Jain
Purification, crystallization ...
Thalictrum flavum
Acta Crystallogr. Sect. F
64
1066-1069
2008
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