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Literature summary for 2.1.1.293 extracted from

  • Shah, G.R.; Wesener, S.R.; Cheng, Y.Q.
    Engineered production of tryprostatins in E. coli through reconstitution of a partial ftm biosynthetic gene cluster from Aspergillus sp. (2015), Jacobs J. Biotechnol. Bioeng., 2, 009.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.293

Cloned(Commentary)

Cloned (Comment) Organism
gene ftmD, encoded in the ftm gene cluster, recombinant expression in Escherichia coli strain BL21(DE3) Aspergillus fumigatus

Protein Variants

Protein Variants Comment Organism
additional information engineered production of tryprostatins in Escherichia coli through reconstitution of a partial ftm biosynthetic gene cluster from Aspergillus sp., i.e. four genes, ftmABCD, on two compatible plasmids Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 6-hydroxytryprostatin B Aspergillus fumigatus
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 S-adenosyl-L-homocysteine + tryprostatin A
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q4WAW6 Af293 R202L variant; isolates Af293, A1163 and BM939, gene ftmD
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 6-hydroxytryprostatin B
-
 Aspergillus fumigatus S-adenosyl-L-homocysteine + tryprostatin A
-
 ?

Synonyms

Synonyms Comment Organism
ftmD
-
 Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Aspergillus fumigatus

General Information

General Information Comment Organism
evolution all ftm genes are expressed almost equally well in both Af293 and BM939 strains. But a point mutation found in ftmD in the genome of Af293 causes an arginine to leucine substitution at position 202 of FtmD, resulting in a dramatic decrease of the catalytic efficiency of FtmD. This mutated form of FtmD appeared not functioning under physiological conditions in Af293 to produce any detectable levels of TPS-A or any downstream metabolites Aspergillus fumigatus